Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
24
pubmed:dateCreated
1997-7-17
pubmed:abstractText
We have analysed the relationship of the products of two genes, neu and BRCA1, known to be important in human breast cancer. Highly specific antibodies that recognized both the rodent and human form of the BRCA1 gene product (Mr 215 kDa, p215BRCA1) were developed to facilitate these efforts. p215BRCA1 was identified as a tyrosine phosphorylated protein primarily localized in the nucleus of several breast cancer cell lines. In transformed murine and human cells, levels of p215BRCA1 tyrosine phosphorylation were inversely correlated with the activity of the erbB family receptor-tyrosine-kinases and with the transformed growth features of these cells. Regulation of p215BRCA1 tyrosine phosphorylation was also related to events in the cell cycle. Increased levels of p215BRCA1 phosphotyrosine content were observed in NIH3T3 cells arrested at the G2/M transition. These findings indicate that the products of BRCA1, neu, and erbB breast cancer genes participate in a common or shared signaling pathway important in cell growth and its regulation.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0950-9232
pubmed:author
pubmed:issnType
Print
pubmed:day
19
pubmed:volume
14
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2863-9
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:9205094-3T3 Cells, pubmed-meshheading:9205094-Amino Acid Sequence, pubmed-meshheading:9205094-Animals, pubmed-meshheading:9205094-Antibody Specificity, pubmed-meshheading:9205094-BRCA1 Protein, pubmed-meshheading:9205094-Breast Neoplasms, pubmed-meshheading:9205094-Cell Cycle, pubmed-meshheading:9205094-Cell Transformation, Neoplastic, pubmed-meshheading:9205094-Humans, pubmed-meshheading:9205094-Mice, pubmed-meshheading:9205094-Molecular Sequence Data, pubmed-meshheading:9205094-Phenotype, pubmed-meshheading:9205094-Phosphorylation, pubmed-meshheading:9205094-Phosphotyrosine, pubmed-meshheading:9205094-Protein-Tyrosine Kinases, pubmed-meshheading:9205094-Receptor Protein-Tyrosine Kinases, pubmed-meshheading:9205094-Signal Transduction, pubmed-meshheading:9205094-Subcellular Fractions, pubmed-meshheading:9205094-Tumor Cells, Cultured, pubmed-meshheading:9205094-Tyrosine
pubmed:year
1997
pubmed:articleTitle
Relationship of p215BRCA1 to tyrosine kinase signaling pathways and the cell cycle in normal and transformed cells.
pubmed:affiliation
Department of Pharmacology, School of Medicine, University of Pennsylvania, Philadelphia 19104, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't