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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1997-7-16
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pubmed:abstractText |
An ELISA was developed for detection of antibodies to GB virus C (GBV-C) using a recombinant E2 protein expressed in CHO cells. Seroconversion to anti-E2 positivity was noted among several persons infected with GBV-C RNA-positive blood through transfusion. Of 6 blood recipients infected by GBV-C RNA-positive donors, 4 (67%) became anti-E2 positive and cleared their viremia. Thus, anti-E2 seroconversion is associated with viral clearance. The prevalence of antibodies to E2 was relatively low (3.0%-8.1%) in volunteer blood donors but was higher in several other groups, including plasmapheresis donors (34.0%), intravenous drug users (85.2%), and West African subjects (13.3%), all of whom tested negative by GBV-C reverse-transcription polymerase chain reaction (RT-PCR). These data demonstrate that testing for anti-E2 should greatly extend the ability of RT-PCR to define the epidemiology and clinical significance of GBV-C.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0022-1899
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pubmed:author |
pubmed-author:AachR DRD,
pubmed-author:BarbosaL HLH,
pubmed-author:CarrickR JRJ,
pubmed-author:ColemanP FPF,
pubmed-author:DawsonG JGJ,
pubmed-author:DilleB JBJ,
pubmed-author:GutierrezR ARA,
pubmed-author:HollingerF BFB,
pubmed-author:KniggeM FMF,
pubmed-author:MosleyJ WJW,
pubmed-author:MushahwarI KIK,
pubmed-author:NemoG JGJ,
pubmed-author:StevensC ECE,
pubmed-author:SurowyT KTK
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pubmed:issnType |
Print
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pubmed:volume |
175
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
458-61
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pubmed:dateRevised |
2005-12-23
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pubmed:meshHeading |
pubmed-meshheading:9203673-Africa,
pubmed-meshheading:9203673-Animals,
pubmed-meshheading:9203673-Antibodies, Viral,
pubmed-meshheading:9203673-Blood Donors,
pubmed-meshheading:9203673-Blood Transfusion,
pubmed-meshheading:9203673-CHO Cells,
pubmed-meshheading:9203673-Cricetinae,
pubmed-meshheading:9203673-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:9203673-Flaviviridae,
pubmed-meshheading:9203673-Hepatitis, Viral, Human,
pubmed-meshheading:9203673-Humans,
pubmed-meshheading:9203673-Plasmapheresis,
pubmed-meshheading:9203673-Polymerase Chain Reaction,
pubmed-meshheading:9203673-Prevalence,
pubmed-meshheading:9203673-RNA, Viral,
pubmed-meshheading:9203673-Recombinant Proteins,
pubmed-meshheading:9203673-Substance Abuse, Intravenous,
pubmed-meshheading:9203673-Viral Envelope Proteins
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pubmed:year |
1997
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pubmed:articleTitle |
An ELISA for detection of antibodies to the E2 protein of GB virus C.
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pubmed:affiliation |
Viral Discovery Group, Abbott Laboratories, North Chicago, Illinois 60064, USA.
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pubmed:publicationType |
Journal Article
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