Linked Life Data

92028

Source:http://linkedlifedata.com/resource/pubmed/id/92028

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
1980-1-24
pubmed:abstractText
A discrete, 600-nucleotide-long plus-strand DNA has been identified among the products of reverse transcription by virions of Moloney murine leukemia virus. Its polarity was shown by hybridization to minus-strand DNA. It appears to be copied from the right end of minus-strand DNA because (i) its restriction endonuclease cleavage pattern corresponds to the redundant 600-base segment found at either end of the ultimate double-stranded reverse transcription products, (ii) its synthesis is actinomycin D sensitive, and (iii) its synthesis begins during the first hour of a reverse transcription reaction when only the right-hand end of minus-strand DNA is available as template. We therefore call this DNA plus-strong-stop DNA by analogy with the minus-strong-stop DNA copied from the left end of the viral RNA. Both strong-stop DNAs are made early during in vitro reactions and decline in concentration later, consistent with postulated roles as initiators of long minus- and plus-strand DNA. Unlike minus-strong-stop DNA, plus-strong-stop DNA remains as a double-stranded nucleic acid after its synthesis, as shown by S1 nuclease resistance. A primer to initiate plus-strong-stop DNA synthesis has not been identified; the product found thus far has no detectable RNA attached to it.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/92028-1188358, http://linkedlifedata.com/resource/pubmed/commentcorrection/92028-13958266, http://linkedlifedata.com/resource/pubmed/commentcorrection/92028-205652, http://linkedlifedata.com/resource/pubmed/commentcorrection/92028-215324, http://linkedlifedata.com/resource/pubmed/commentcorrection/92028-215781, http://linkedlifedata.com/resource/pubmed/commentcorrection/92028-265521, http://linkedlifedata.com/resource/pubmed/commentcorrection/92028-509527, http://linkedlifedata.com/resource/pubmed/commentcorrection/92028-61277, http://linkedlifedata.com/resource/pubmed/commentcorrection/92028-657274, http://linkedlifedata.com/resource/pubmed/commentcorrection/92028-77733, http://linkedlifedata.com/resource/pubmed/commentcorrection/92028-88264, http://linkedlifedata.com/resource/pubmed/commentcorrection/92028-90578
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:volume
76
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4355-9
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1979
pubmed:articleTitle
Synthesis of a 600-nucleotide-long plus-strand DNA by virions of Moloney murine leukemia virus.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.