Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
27
pubmed:dateCreated
1997-7-31
pubmed:abstractText
The proliferation of vascular endothelial cells (EC) is an important event in angiogenesis. The synthesis of the EC growth factor, vascular endothelial cell growth factor (VEGF), is stimulated by a variety of activators; but the effects of important vasoactive peptides are not well understood, and there are no known natural inhibitors of VEGF production. We found that the vasoactive peptides endothelin (ET)-1 and ET-3 stimulated the synthesis of VEGF protein 3-4-fold in cultured human vascular smooth muscle cells, comparable in magnitude to hypoxia. ET-1 and ET-3 acted through the ETA and ETB receptors, respectively, and signaling through protein kinase C was important. Atrial natriuretic peptide (ANP), C-type natriuretic peptide, and C-ANP-(4-23), a ligand for the natriuretic peptide clearance receptor, equipotently inhibited production of VEGF by as much as 88% and inhibited ET- or hypoxia-stimulated VEGF transcription. EC proliferation and invasion of matrix were stimulated by VEGF secreted into the medium by ET-incubated vascular smooth muscle cells. This was inhibited by ANP. Our results identify the natriuretic peptides as the first peptide inhibitors of VEGF synthesis and indicate a novel mechanism by which vasoactive peptides could modulate angiogenesis.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Atrial Natriuretic Factor, http://linkedlifedata.com/resource/pubmed/chemical/Endothelial Growth Factors, http://linkedlifedata.com/resource/pubmed/chemical/Endothelin-1, http://linkedlifedata.com/resource/pubmed/chemical/Endothelin-3, http://linkedlifedata.com/resource/pubmed/chemical/GTP-Binding Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Lymphokines, http://linkedlifedata.com/resource/pubmed/chemical/Natriuretic Peptide, C-Type, http://linkedlifedata.com/resource/pubmed/chemical/Protein Sorting Signals, http://linkedlifedata.com/resource/pubmed/chemical/Proteins, http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger, http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Endothelin, http://linkedlifedata.com/resource/pubmed/chemical/Vascular Endothelial Growth Factor A, http://linkedlifedata.com/resource/pubmed/chemical/Vascular Endothelial Growth Factors
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
4
pubmed:volume
272
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
17097-103
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:9202027-Animals, pubmed-meshheading:9202027-Atrial Natriuretic Factor, pubmed-meshheading:9202027-Cattle, pubmed-meshheading:9202027-Cell Division, pubmed-meshheading:9202027-Endothelial Growth Factors, pubmed-meshheading:9202027-Endothelin-1, pubmed-meshheading:9202027-Endothelin-3, pubmed-meshheading:9202027-Endothelium, Vascular, pubmed-meshheading:9202027-GTP-Binding Proteins, pubmed-meshheading:9202027-Humans, pubmed-meshheading:9202027-Lymphokines, pubmed-meshheading:9202027-Muscle, Smooth, Vascular, pubmed-meshheading:9202027-Natriuretic Peptide, C-Type, pubmed-meshheading:9202027-Neovascularization, Physiologic, pubmed-meshheading:9202027-Protein Biosynthesis, pubmed-meshheading:9202027-Protein Sorting Signals, pubmed-meshheading:9202027-Proteins, pubmed-meshheading:9202027-RNA, Messenger, pubmed-meshheading:9202027-Receptors, Endothelin, pubmed-meshheading:9202027-Transcription, Genetic, pubmed-meshheading:9202027-Vascular Endothelial Growth Factor A, pubmed-meshheading:9202027-Vascular Endothelial Growth Factors
pubmed:year
1997
pubmed:articleTitle
Vasoactive peptides modulate vascular endothelial cell growth factor production and endothelial cell proliferation and invasion.
pubmed:affiliation
Department of Medicine, University of California, Irvine, California 92717, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.