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rdf:type |
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lifeskim:mentions |
umls-concept:C0015283,
umls-concept:C0017337,
umls-concept:C0020792,
umls-concept:C0025246,
umls-concept:C0030448,
umls-concept:C0205245,
umls-concept:C0314603,
umls-concept:C0449445,
umls-concept:C0851285,
umls-concept:C1514873,
umls-concept:C1546857,
umls-concept:C1556066,
umls-concept:C1619636
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pubmed:issue |
6
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pubmed:dateCreated |
1997-8-26
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pubmed:abstractText |
Paramecium is a unicellular organism that possesses a specialized pathway for regulated secretion that is amenable to genetic studies. Numerous mutations affecting the process have been isolated over the years, among which is a subclass blocking the terminal step of fusion of the secretory granule with the plasma membrane. We report herein the cloning by functional complementation of one such gene, ND7. The 506-amino acid polypeptide encoded by ND7 is predicted to be a type I integral membrane protein with a highly charged cytosolic domain featuring amphipathic and coiled-coil regions. This structure is compatible with the physiological data on the mutant nd7-1 suggesting that the protein is anchored in the membrane of the secretory granule and that it may interact with other proteins. This work presents the first identification by a genetic approach of a novel gene involved in regulated secretion and establishes Paramecium as a powerful model system for the genetic dissection of this process.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/9201716-1254639,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9201716-1339345,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9201716-1453356,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9201716-1551571,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9201716-1703537,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9201716-17797907,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9201716-1851293,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9201716-2163766,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9201716-2186516,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9201716-2231712,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9201716-2823267,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9201716-2832831,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9201716-3024967,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9201716-38256,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9201716-395132,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9201716-3974721,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9201716-3974722,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9201716-3979401,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9201716-455408,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9201716-4772364,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9201716-4837325,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9201716-6546423,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9201716-7203007,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9201716-7204496,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9201716-7372705,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9201716-7400221,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9201716-7777058,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9201716-7791897,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9201716-7969419,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9201716-8096639,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9201716-8126732,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9201716-8165136,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9201716-8253066,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9201716-8343109,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9201716-8345525,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9201716-8391930,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9201716-8435773
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
1059-1524
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pubmed:author |
|
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pubmed:issnType |
Print
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pubmed:volume |
8
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1063-71
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pubmed:dateRevised |
2010-9-13
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pubmed:meshHeading |
pubmed-meshheading:9201716-Amino Acid Sequence,
pubmed-meshheading:9201716-Animals,
pubmed-meshheading:9201716-Base Sequence,
pubmed-meshheading:9201716-Cytoplasm,
pubmed-meshheading:9201716-Exocytosis,
pubmed-meshheading:9201716-Genes, Protozoan,
pubmed-meshheading:9201716-Genetic Complementation Test,
pubmed-meshheading:9201716-Membrane Fusion,
pubmed-meshheading:9201716-Membrane Proteins,
pubmed-meshheading:9201716-Molecular Sequence Data,
pubmed-meshheading:9201716-Paramecium tetraurelia,
pubmed-meshheading:9201716-Protozoan Proteins,
pubmed-meshheading:9201716-Restriction Mapping
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pubmed:year |
1997
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pubmed:articleTitle |
Genetic approach to regulated exocytosis using functional complementation in Paramecium: identification of the ND7 gene required for membrane fusion.
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pubmed:affiliation |
Centre de Génétique Moléculaire, Centre National de la Recherche Scientifique, Gif-sur-Yvette, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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