Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1997-8-27
pubmed:abstractText
Bends in nucleic acid helices can be quantified in a transient electric birefringence (TEB) experiment from the ratio of the terminal decay times of the bent molecule and its fully duplex counterpart (tau-ratio method). The apparent bend angles can be extracted from the experimental tau-ratios through the application of static (equilibrium-ensemble) hydrodynamic models; however, such models do not properly address the faster component(s) of the birefringence decay profile, which can represent up to 80% of the total birefringence signal for large band angles. To address this latter issue, the relative amplitudes of the components in the birefringence decay profile have been analyzed through a series of Brownian dynamics (BD) simulations. Decay profiles have been simulated for three-, five-, and nine-bead models representing RNA molecules with central bends of 30 degrees, 60 degrees, and 90 degrees, and with various degrees of associated angle dispersion. The BD simulations are in close agreement with experimental results for the fractional amplitudes, suggesting that both amplitudes and terminal tau-ratios can be used as a measure of the magnitudes of bends in the helix axis. Although the current results indicate that it is generally not possible to distinguish between relatively fixed and highly flexible bends from single tau-ratio measurements, because they can lead to similar reductions in terminal decay time and amplitude, measurements of the dependence of the fractional amplitudes on helix length may afford such a distinction.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/9199796-2021619, http://linkedlifedata.com/resource/pubmed/commentcorrection/9199796-3233330, http://linkedlifedata.com/resource/pubmed/commentcorrection/9199796-3589666, http://linkedlifedata.com/resource/pubmed/commentcorrection/9199796-6260262, http://linkedlifedata.com/resource/pubmed/commentcorrection/9199796-6288135, http://linkedlifedata.com/resource/pubmed/commentcorrection/9199796-7023566, http://linkedlifedata.com/resource/pubmed/commentcorrection/9199796-7509191, http://linkedlifedata.com/resource/pubmed/commentcorrection/9199796-7520504, http://linkedlifedata.com/resource/pubmed/commentcorrection/9199796-7535562, http://linkedlifedata.com/resource/pubmed/commentcorrection/9199796-7536250, http://linkedlifedata.com/resource/pubmed/commentcorrection/9199796-7597079, http://linkedlifedata.com/resource/pubmed/commentcorrection/9199796-7669910, http://linkedlifedata.com/resource/pubmed/commentcorrection/9199796-7761403, http://linkedlifedata.com/resource/pubmed/commentcorrection/9199796-7947724, http://linkedlifedata.com/resource/pubmed/commentcorrection/9199796-8268160, http://linkedlifedata.com/resource/pubmed/commentcorrection/9199796-8609623, http://linkedlifedata.com/resource/pubmed/commentcorrection/9199796-8757281, http://linkedlifedata.com/resource/pubmed/commentcorrection/9199796-8764401, http://linkedlifedata.com/resource/pubmed/commentcorrection/9199796-8913687, http://linkedlifedata.com/resource/pubmed/commentcorrection/9199796-9199795
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0006-3495
pubmed:author
pubmed:issnType
Print
pubmed:volume
73
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
318-32
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1997
pubmed:articleTitle
Influence of static and dynamic bends on the birefringence decay profile of RNA helices: Brownian dynamics simulations.
pubmed:affiliation
Department of Biochemistry and Molecular Genetics, University of Colorado Health Sciences Center, Denver 80262, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't