Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
1997-7-21
pubmed:abstractText
Clavanins are histidine-rich, amidated alpha-helical antimicrobial peptides that were originally isolated from the leukocytes (hemocytes) of a tunicate, Styela clava. The activities of clavanin A amide and clavanin A acid against Escherichia coli, Listeria monocytogenes, and Candida albicans were substantially greater at pH 5.5 than at pH 7.4. In contrast, clavanin AK, a synthetic variant of clavanin A acid containing 4 histidine-->lysine substitutions exerted substantial activity at both pH 7.4 and pH 5.5. Each of these three clavanins permeabilized the outer and inner membranes of E. coli very effectively at pH 5.5, but only clavanin AK did so at pH 7.4. Unlike magainin 1 and cecropin P1, alpha-helical antimicrobial peptides from frog skin and porcine intestine, respectively, clavanins were broadly effective against gram-positive bacteria, including methicillin-resistant Staphylococcus aureus, as well as gram-negative organisms. Because clavanins exert substantial antimicrobial activity in 0.1 to 0.3 M NaCl, they provide templates for designing broad-spectrum peptide antibiotics intended to function in extracellular environments containing normal or elevated NaCl concentrations. The pH-dependent properties of histidine-rich antimicrobial peptides may allow the design of agents that would function selectively in acidic compartments, such as the gastric lumen, or within phagolysosomes.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/9199465-1510920, http://linkedlifedata.com/resource/pubmed/commentcorrection/9199465-1855975, http://linkedlifedata.com/resource/pubmed/commentcorrection/9199465-1901580, http://linkedlifedata.com/resource/pubmed/commentcorrection/9199465-2317507, http://linkedlifedata.com/resource/pubmed/commentcorrection/9199465-2481510, http://linkedlifedata.com/resource/pubmed/commentcorrection/9199465-2512577, http://linkedlifedata.com/resource/pubmed/commentcorrection/9199465-2668334, http://linkedlifedata.com/resource/pubmed/commentcorrection/9199465-270513, http://linkedlifedata.com/resource/pubmed/commentcorrection/9199465-2719677, http://linkedlifedata.com/resource/pubmed/commentcorrection/9199465-2719968, http://linkedlifedata.com/resource/pubmed/commentcorrection/9199465-2773933, http://linkedlifedata.com/resource/pubmed/commentcorrection/9199465-2833514, http://linkedlifedata.com/resource/pubmed/commentcorrection/9199465-3127470, http://linkedlifedata.com/resource/pubmed/commentcorrection/9199465-3277183, http://linkedlifedata.com/resource/pubmed/commentcorrection/9199465-3286634, http://linkedlifedata.com/resource/pubmed/commentcorrection/9199465-3299384, http://linkedlifedata.com/resource/pubmed/commentcorrection/9199465-3606567, http://linkedlifedata.com/resource/pubmed/commentcorrection/9199465-4197885, http://linkedlifedata.com/resource/pubmed/commentcorrection/9199465-6724692, http://linkedlifedata.com/resource/pubmed/commentcorrection/9199465-6724693, http://linkedlifedata.com/resource/pubmed/commentcorrection/9199465-7140755, http://linkedlifedata.com/resource/pubmed/commentcorrection/9199465-7498547, http://linkedlifedata.com/resource/pubmed/commentcorrection/9199465-7547876, http://linkedlifedata.com/resource/pubmed/commentcorrection/9199465-7548003, http://linkedlifedata.com/resource/pubmed/commentcorrection/9199465-7589491, http://linkedlifedata.com/resource/pubmed/commentcorrection/9199465-7612236, http://linkedlifedata.com/resource/pubmed/commentcorrection/9199465-7621828, http://linkedlifedata.com/resource/pubmed/commentcorrection/9199465-7642143, http://linkedlifedata.com/resource/pubmed/commentcorrection/9199465-7659744, http://linkedlifedata.com/resource/pubmed/commentcorrection/9199465-7726268, http://linkedlifedata.com/resource/pubmed/commentcorrection/9199465-7893944, http://linkedlifedata.com/resource/pubmed/commentcorrection/9199465-7946046, http://linkedlifedata.com/resource/pubmed/commentcorrection/9199465-8136371, http://linkedlifedata.com/resource/pubmed/commentcorrection/9199465-8144548, http://linkedlifedata.com/resource/pubmed/commentcorrection/9199465-8192325, http://linkedlifedata.com/resource/pubmed/commentcorrection/9199465-8335113, http://linkedlifedata.com/resource/pubmed/commentcorrection/9199465-8506327, http://linkedlifedata.com/resource/pubmed/commentcorrection/9199465-8517799, http://linkedlifedata.com/resource/pubmed/commentcorrection/9199465-8612275, http://linkedlifedata.com/resource/pubmed/commentcorrection/9199465-8708129, http://linkedlifedata.com/resource/pubmed/commentcorrection/9199465-8769093
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0019-9567
pubmed:author
pubmed:issnType
Print
pubmed:volume
65
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2898-903
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1997
pubmed:articleTitle
Effects of pH and salinity on the antimicrobial properties of clavanins.
pubmed:affiliation
Department of Medicine, UCLA School of Medicine, Los Angeles, California 90095-1690, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.