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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1997-7-24
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pubmed:databankReference |
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pubmed:abstractText |
Dopamine D4 receptor (DRD4) has received attention in terms of pathogenesis of schizophrenia and association with human personalities. We isolated the human DRD4 gene containing the 5'-flanking region and determined its sequence. Analysis of the DRD4 transcripts by 5'-RACE (5'-rapid amplification of cDNA ends) revealed a region of the transcription initiation located between -501 and -436 relative to the first nucleotide of the initiation codon. There is a CpG island spanning from -900 to +500 but no TATA or CAAT boxes in the 5'-flanking region. Functional analysis of the 5'-flanking region of the DRD4 gene by a transient expression method revealed the presence of a negative modulator between -770 and -679. The region between -591 and -123 gave the highest transcriptional activity in IMR32 (neuroblastoma) and Y-79 (retinoblastoma) cells but not in HeLa cells, suggesting that this housekeeping gene-like promoter regulates the cell-type specific gene expression.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
18
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pubmed:volume |
235
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
321-6
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pubmed:dateRevised |
2005-11-17
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pubmed:meshHeading |
pubmed-meshheading:9199190-Base Sequence,
pubmed-meshheading:9199190-Chloramphenicol O-Acetyltransferase,
pubmed-meshheading:9199190-Cloning, Molecular,
pubmed-meshheading:9199190-CpG Islands,
pubmed-meshheading:9199190-Exons,
pubmed-meshheading:9199190-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:9199190-HeLa Cells,
pubmed-meshheading:9199190-Humans,
pubmed-meshheading:9199190-Molecular Sequence Data,
pubmed-meshheading:9199190-Neuroblastoma,
pubmed-meshheading:9199190-Polymerase Chain Reaction,
pubmed-meshheading:9199190-RNA, Messenger,
pubmed-meshheading:9199190-Receptors, Dopamine D2,
pubmed-meshheading:9199190-Receptors, Dopamine D4,
pubmed-meshheading:9199190-Regulatory Sequences, Nucleic Acid,
pubmed-meshheading:9199190-Repetitive Sequences, Nucleic Acid,
pubmed-meshheading:9199190-Retinoblastoma,
pubmed-meshheading:9199190-Sequence Analysis, DNA,
pubmed-meshheading:9199190-Tumor Cells, Cultured
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pubmed:year |
1997
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pubmed:articleTitle |
Cloning and characterization of the 5'-flanking region of the human dopamine D4 receptor gene.
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pubmed:affiliation |
Institute of Genetic Information, Kyushu University, Fukuoka, Japan.
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pubmed:publicationType |
Journal Article
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