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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1997-7-21
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pubmed:databankReference |
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pubmed:abstractText |
We report the cloning and functional characterization of the iron responsive element (IRE) of human ferritin light (L) chain mRNA from a cDNA library of primary human T lymphocytes. Comparison of this palindromic cDNA element to the IRE predicted from the reported genomic sequence revealed significant differences, resulting in a stem-loop structure with lower stability than the IRE of the heavy (H) chain mRNA. Nevertheless, the L subunit IRE mediated efficient binding of the iron regulatory protein (IRP) in a manner comparable to that of human ferritin H chain mRNA in vitro. In accordance with previous observations on H form transcripts, the cis-acting regulatory IRE motif of human ferritin L chain mRNA was capable of repressing translation under iron deprivation but permitted mobilization of the transcripts into polysomes following iron repletion in vivo.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
9
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pubmed:volume |
235
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
212-6
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:9196065-Base Sequence,
pubmed-meshheading:9196065-Blotting, Northern,
pubmed-meshheading:9196065-Cloning, Molecular,
pubmed-meshheading:9196065-DNA, Complementary,
pubmed-meshheading:9196065-DNA Probes,
pubmed-meshheading:9196065-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:9196065-Ferritins,
pubmed-meshheading:9196065-HeLa Cells,
pubmed-meshheading:9196065-Humans,
pubmed-meshheading:9196065-Iron,
pubmed-meshheading:9196065-Iron-Regulatory Proteins,
pubmed-meshheading:9196065-Iron-Sulfur Proteins,
pubmed-meshheading:9196065-Molecular Sequence Data,
pubmed-meshheading:9196065-Nucleic Acid Conformation,
pubmed-meshheading:9196065-RNA, Messenger,
pubmed-meshheading:9196065-RNA-Binding Proteins,
pubmed-meshheading:9196065-Sequence Homology, Nucleic Acid,
pubmed-meshheading:9196065-T-Lymphocytes
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pubmed:year |
1997
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pubmed:articleTitle |
Structure and function of the iron-responsive element from human ferritin L chain mRNA.
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pubmed:affiliation |
Institute of Molecular Biology, Vienna Biocenter, University of Vienna, Austria.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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