|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
4
|
|
pubmed:dateCreated |
1997-7-29
|
|
pubmed:abstractText |
Fluorescence detection of cell membrane potentials is an important technique in neurobiology, cell physiology and pharmaceutical screening, but traditional one-fluorophore indicators either respond too slowly or have limited sensitivity. Recently, we introduced two-component sensors based on the transfer of fluorescence resonance energy from fluorescent lectins bound on one side of the plasma membrane to highly fluorescent oxonol acceptors that electrophorese from one face of the membrane to the other in response to membrane potential.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Apr
|
|
pubmed:issn |
1074-5521
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
4
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
269-77
|
|
pubmed:dateRevised |
2006-11-15
|
|
pubmed:meshHeading |
pubmed-meshheading:9195864-Animals,
pubmed-meshheading:9195864-Cell Line,
pubmed-meshheading:9195864-Coumarins,
pubmed-meshheading:9195864-Energy Transfer,
pubmed-meshheading:9195864-Fluorescence,
pubmed-meshheading:9195864-Fluorescent Dyes,
pubmed-meshheading:9195864-Kinetics,
pubmed-meshheading:9195864-Lipid Bilayers,
pubmed-meshheading:9195864-Mammals,
pubmed-meshheading:9195864-Membrane Potentials,
pubmed-meshheading:9195864-Patch-Clamp Techniques,
pubmed-meshheading:9195864-Phosphatidylethanolamines,
pubmed-meshheading:9195864-Photochemistry,
pubmed-meshheading:9195864-Spectrometry, Fluorescence,
pubmed-meshheading:9195864-Thiobarbiturates,
pubmed-meshheading:9195864-Tumor Cells, Cultured,
pubmed-meshheading:9195864-Xanthophylls,
pubmed-meshheading:9195864-beta Carotene
|
|
pubmed:year |
1997
|
|
pubmed:articleTitle |
Improved indicators of cell membrane potential that use fluorescence resonance energy transfer.
|
|
pubmed:affiliation |
Howard Hughes Medical Institute and Department of Pharmacology, 310 Cellular and Molecular Medicine West, University of California, 9500 Gilman Drive, CA 92093-0647, USA.
|
|
pubmed:publicationType |
Journal Article
|
