Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
11
|
| pubmed:dateCreated |
1997-7-24
|
| pubmed:abstractText |
T cell depletion using the murine monoclonal antibody (moAb) T10B9 is unique in that the T cell receptor (TCR)gamma delta bearing subset is relatively spared compared to the TCR alpha beta + subset. We evaluated the probabilities of engraftment, acute and chronic graft-versus-host disease (GVHD), relapse, and survival in 273 recipients of marrow T cell depleted using T10B9. Sixty-two patients received marrow from an HLA-identical sibling, 54 patients received partially matched related donor marrow and 157 patients received unrelated donor marrow. Limiting dilution analysis (LDA) was used to estimate total clonable T cell dose in all patients and a modified LDA using moAb-coated immunomagnetic beads was used to estimate TCR alpha beta +, CD4+, and CD8+ T cells in a subset of patients. TCR gamma delta + cell dose was estimated by flow cytometry. Cox proportional hazards regression models were used to assess the impact of T cell subset dose/kg of body weight on outcome. We found a significant association of TCR gamma delta + T cell dose (P = 0.004), but not TCR alpha beta + T cell dose or total clonable T cell dose, with the probability of engraftment. TCR alpha beta +, CD4+, CD8+ and total clonable T cell dose were significantly associated (P < 0.001) with the risks of grade 2-4 acute GVHD in recipients of marrow from related donors but not in recipients of marrow from unrelated donors. Neither total clonable T cell dose nor any T cell subset dose was found to be significantly associated with chronic GVHD, relapse or survival. The results confirm preclinical studies showing TCR gamma delta + T cells promote engraftment. TCR gamma delta + T cells are not associated with an increased risk of acute GVHD while TCR alpha beta T cells are associated with acute GVHD but not engraftment in recipients of marrow grafts T cell depleted using T10B9. These findings support the hypothesis that T cell subsets differentially contribute to alloengraftment and GVHD.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jun
|
| pubmed:issn |
0268-3369
|
| pubmed:author |
pubmed-author:CamittaBB,
pubmed-author:CasperJJ,
pubmed-author:Cook-CraigAA,
pubmed-author:DrobyskiW RWR,
pubmed-author:FlomenbergNN,
pubmed-author:GarbrechtFF,
pubmed-author:HorowitzMM,
pubmed-author:JuckettMM,
pubmed-author:KawanishiYY,
pubmed-author:Keever-TaylorC ACA,
pubmed-author:MargolisDD,
pubmed-author:PasswegJJ,
pubmed-author:PietrygaDD,
pubmed-author:RowlingsPP
|
| pubmed:issnType |
Print
|
| pubmed:volume |
19
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1069-77
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:9193748-Adolescent,
pubmed-meshheading:9193748-Adult,
pubmed-meshheading:9193748-Aged,
pubmed-meshheading:9193748-Bone Marrow Transplantation,
pubmed-meshheading:9193748-Child,
pubmed-meshheading:9193748-Child, Preschool,
pubmed-meshheading:9193748-Graft vs Host Disease,
pubmed-meshheading:9193748-Humans,
pubmed-meshheading:9193748-Infant,
pubmed-meshheading:9193748-Lymphocyte Depletion,
pubmed-meshheading:9193748-Middle Aged,
pubmed-meshheading:9193748-Multivariate Analysis,
pubmed-meshheading:9193748-Receptors, Antigen, T-Cell, alpha-beta,
pubmed-meshheading:9193748-Receptors, Antigen, T-Cell, gamma-delta,
pubmed-meshheading:9193748-T-Lymphocyte Subsets
|
| pubmed:year |
1997
|
| pubmed:articleTitle |
Effect of T cell subset dose on outcome of T cell-depleted bone marrow transplantation.
|
| pubmed:affiliation |
Bone Marrow Transplantation Program of the Medical College of Wisconsin, Milwaukee 53226, USA.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|