9193080

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Predicate	Object
rdf:type	pubmed:Citation
lifeskim:mentions	umls-concept:C0006556, umls-concept:C0017337, umls-concept:C0020792, umls-concept:C0035552, umls-concept:C0162326, umls-concept:C0162740, umls-concept:C0441513, umls-concept:C0521449, umls-concept:C0679058, umls-concept:C0680022, umls-concept:C0728938, umls-concept:C1511491, umls-concept:C1517488, umls-concept:C1527177, umls-concept:C1547699, umls-concept:C2700640
pubmed:issue	5
pubmed:dateCreated	1997-8-5
pubmed:databankReference	http://linkedlifedata.com/resource/pubmed/xref/GENBANK/A34571, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/A36571, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/B24028, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/C36571, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/D38010, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/L27461, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/L31645, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/M62396, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/S11393, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/S19164, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/S22789, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/S32578, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/S39486, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/S42260, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/S51347, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U10046, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U30454, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U30495, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X77456, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X91958, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X91959, http://linkedlifedata.com/resource/pubmed/xref/SWISSPROT/P17094, http://linkedlifedata.com/resource/pubmed/xref/SWISSPROT/P23358, http://linkedlifedata.com/resource/pubmed/xref/SWISSPROT/P29766, http://linkedlifedata.com/resource/pubmed/xref/SWISSPROT/P35685, http://linkedlifedata.com/resource/pubmed/xref/SWISSPROT/P38666, http://linkedlifedata.com/resource/pubmed/xref/SWISSPROT/P41099, http://linkedlifedata.com/resource/pubmed/xref/SWISSPROT/P41127, http://linkedlifedata.com/resource/pubmed/xref/SWISSPROT/P46286, http://linkedlifedata.com/resource/pubmed/xref/SWISSPROT/Q07760
pubmed:abstractText	Partial cDNA sequencing to obtain expressed sequence tags (ESTs) has led to the identification of tags to about 8,000 of the estimated 20,000 genes on Arabidopsis thaliana. This figure represents four to five times the number of complete coding sequences from this organism available in international databases. In contrast to mammals, many proteins are encoded by multigene families in A. thaliana. Using ribosomal protein gene families as an example, it is possible to construct relatively long sequences from overlapping ESTs which are of sufficiently high quality to be able to unambiguously identify tags to individual members of multigene families, even when the sequences are highly conserved. A total of 106 genes encoding 50 different cytoplasmic ribosomal protein types have been identified, most proteins being encoded by at least two and up to four genes. Coding sequences of members of individual gene families are almost always very highly conserved and derived amino acid sequences are almost, if not completely, identical in the vast majority of cases. Sequence divergence is observed in untranslated regions which allows the definition of gene-specific probes. The method can be used to construct high-quality tags to any protein.
pubmed:language	eng
pubmed:journal	http://linkedlifedata.com/resource/pubmed/journal/9207397
pubmed:citationSubset	IM
pubmed:chemical	http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary, http://linkedlifedata.com/resource/pubmed/chemical/Oligonucleotide Probes, http://linkedlifedata.com/resource/pubmed/chemical/Ribosomal Protein S6, http://linkedlifedata.com/resource/pubmed/chemical/Ribosomal Proteins, http://linkedlifedata.com/resource/pubmed/chemical/ribosomal protein L1, http://linkedlifedata.com/resource/pubmed/chemical/ribosomal protein S18
pubmed:status	MEDLINE
pubmed:month	May
pubmed:issn	0960-7412
pubmed:author	pubmed-author:CookeRR, pubmed-author:DelsenyMM, pubmed-author:LaudiéMM, pubmed-author:RaynalMM
pubmed:issnType	Print
pubmed:volume	11
pubmed:owner	NLM
pubmed:authorsComplete	Y
pubmed:pagination	1127-40
pubmed:dateRevised	2006-11-15
pubmed:meshHeading	pubmed-meshheading:9193080-Amino Acid Sequence, pubmed-meshheading:9193080-Arabidopsis, pubmed-meshheading:9193080-Base Sequence, pubmed-meshheading:9193080-Conserved Sequence, pubmed-meshheading:9193080-Cytoplasm, pubmed-meshheading:9193080-DNA, Complementary, pubmed-meshheading:9193080-Databases, Factual, pubmed-meshheading:9193080-Gene Expression, pubmed-meshheading:9193080-Genes, Plant, pubmed-meshheading:9193080-Molecular Sequence Data, pubmed-meshheading:9193080-Multigene Family, pubmed-meshheading:9193080-Oligonucleotide Probes, pubmed-meshheading:9193080-Ribosomal Protein S6, pubmed-meshheading:9193080-Ribosomal Proteins, pubmed-meshheading:9193080-Sequence Alignment, pubmed-meshheading:9193080-Sequence Analysis, DNA, pubmed-meshheading:9193080-Sequence Homology, Amino Acid, pubmed-meshheading:9193080-Sequence Homology, Nucleic Acid, pubmed-meshheading:9193080-Software
pubmed:year	1997
pubmed:articleTitle	Identification of members of gene families in Arabidopsis thaliana by contig construction from partial cDNA sequences: 106 genes encoding 50 cytoplasmic ribosomal proteins.
pubmed:affiliation	Laboratoire de Physiologie et Biologie Moléculaires Végétales, UMR5545 du CNRS, Université de Perpignan, France.
pubmed:publicationType	Journal Article, Comparative Study, Research Support, Non-U.S. Gov't