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rdf:type |
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lifeskim:mentions |
umls-concept:C0017337,
umls-concept:C0024779,
umls-concept:C0075212,
umls-concept:C0085859,
umls-concept:C0205147,
umls-concept:C0205314,
umls-concept:C0679622,
umls-concept:C0751785,
umls-concept:C0949374,
umls-concept:C1511545,
umls-concept:C1514583,
umls-concept:C1521082,
umls-concept:C1857700,
umls-concept:C2700640
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pubmed:issue |
2
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pubmed:dateCreated |
1997-9-2
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pubmed:databankReference |
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pubmed:abstractText |
We have cloned and molecularly characterized a novel human cDNA that encodes a protein of about 52 kDa that was shown by immunocytochemistry to have a nuclear localization. Northern blot analysis of a variety of human tissues revealed that the gene for this novel nuclear protein, designated NNP-1 (HGMW-approved symbol D21S2056E), is ubiquitously expressed as a 2.0-kb transcript. The NNP-1 protein displays significant sequence similarity to the C47E12.7 protein of Caenorhabditis elegans (38% identity and 58% similarity) and the YD78 protein of Saccharomyces cerevisiae (36% identity and 54% similarity). The human NNP-1 gene was mapped to a 15-kb DNA interval on chromosome 21q22.3, close to markers D21S1459 and D21S1953, that is 25 kb distal to the gene encoding cystatin B. Finally, an as-yet unknown gene that is highly related to NNP-1 was found proximal to the gene encoding cystatin B, near marker D21S1458, which is approximately 75 kb centromeric to the NNP-1 gene.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0888-7543
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
42
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
336-41
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:9192856-Amino Acid Sequence,
pubmed-meshheading:9192856-Animals,
pubmed-meshheading:9192856-Base Sequence,
pubmed-meshheading:9192856-COS Cells,
pubmed-meshheading:9192856-Chromosome Mapping,
pubmed-meshheading:9192856-Chromosomes, Human, Pair 21,
pubmed-meshheading:9192856-Cloning, Molecular,
pubmed-meshheading:9192856-Cystatin B,
pubmed-meshheading:9192856-Cystatins,
pubmed-meshheading:9192856-DNA, Complementary,
pubmed-meshheading:9192856-Exons,
pubmed-meshheading:9192856-Genetic Markers,
pubmed-meshheading:9192856-Humans,
pubmed-meshheading:9192856-Introns,
pubmed-meshheading:9192856-Microscopy, Fluorescence,
pubmed-meshheading:9192856-Molecular Sequence Data,
pubmed-meshheading:9192856-Nuclear Proteins,
pubmed-meshheading:9192856-Sequence Homology, Amino Acid,
pubmed-meshheading:9192856-Transfection
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pubmed:year |
1997
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pubmed:articleTitle |
The NNP-1 gene (D21S2056E), which encodes a novel nuclear protein, maps in close proximity to the cystatin B gene within the EPM1 and APECED critical region on 21q22.3.
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pubmed:affiliation |
Laboratory for Molecular Oncology, Center for Human Genetics, University of Leuven, Belgium.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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