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rdf:type |
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lifeskim:mentions |
umls-concept:C0006556,
umls-concept:C0009017,
umls-concept:C0086418,
umls-concept:C0282552,
umls-concept:C0524914,
umls-concept:C0679058,
umls-concept:C1334043,
umls-concept:C1547699,
umls-concept:C1880022,
umls-concept:C2697616,
umls-concept:C2700640
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pubmed:issue |
12
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pubmed:dateCreated |
1997-7-10
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pubmed:databankReference |
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pubmed:abstractText |
Chemokines refer to a rapidly expanding family of small cytokines whose primary function is recruitment of leukocytes to inflammatory sites. These are known to bind to seven-transmembrane-domain containing receptors. A cDNA clone, CHEMR1, resembling the typical G protein-coupled receptor, was isolated from a mouse cytotoxic T-lymphocyte (CTL) library. Northern blot analysis in mouse cell lines suggests that its expression is found in a variety of cells, including T cells, B cells, and macrophages. The CHEMR1 gene Scya3r2 is a single-copy gene whose open reading frame may be in a single exon and maps to the distal region of mouse Chr 9 where the mouse macrophage inflammatory protein-1alpha (MIP-1alpha) receptor gene Scya3r and two related C-C chemokine receptor-like genes reside. Amino acid sequence comparison shows that CHEMR1 is 84% identical to human CCR-4, indicating that CHEMR1 is likely to be a mouse CCR-4. Binding assays using 125I-labeled C-C chemokines in mammalian cells indicated that CHEMR1 did not bind MIP-1alpha, RANTES, or MIP-1beta, whereas CCR-1 binds MIP-1alpha and RANTES. Our result is different from the reported properties of human CCR-4. This suggests that CHEMR1 may be a receptor for unidentified C-C chemokine or a low-affinity receptor for MIP-1alpha.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/CCL17 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/CCR1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/CCR4 protein, S cerevisiae,
http://linkedlifedata.com/resource/pubmed/chemical/Ccr1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Chemokine CCL17,
http://linkedlifedata.com/resource/pubmed/chemical/Chemokine CCL3,
http://linkedlifedata.com/resource/pubmed/chemical/Chemokine CCL4,
http://linkedlifedata.com/resource/pubmed/chemical/Chemokine CCL5,
http://linkedlifedata.com/resource/pubmed/chemical/Chemokines,
http://linkedlifedata.com/resource/pubmed/chemical/Chemokines, CC,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/Fungal Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Macrophage Inflammatory Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, CCR1,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Chemokine,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cytokine,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Ribonucleases,
http://linkedlifedata.com/resource/pubmed/chemical/Saccharomyces cerevisiae Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0006-4971
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
89
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4448-60
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:9192769-Amino Acid Sequence,
pubmed-meshheading:9192769-Animals,
pubmed-meshheading:9192769-Base Sequence,
pubmed-meshheading:9192769-Chemokine CCL17,
pubmed-meshheading:9192769-Chemokine CCL3,
pubmed-meshheading:9192769-Chemokine CCL4,
pubmed-meshheading:9192769-Chemokine CCL5,
pubmed-meshheading:9192769-Chemokines,
pubmed-meshheading:9192769-Chemokines, CC,
pubmed-meshheading:9192769-Chromosome Mapping,
pubmed-meshheading:9192769-Cloning, Molecular,
pubmed-meshheading:9192769-DNA, Complementary,
pubmed-meshheading:9192769-Fungal Proteins,
pubmed-meshheading:9192769-Gene Library,
pubmed-meshheading:9192769-Genes,
pubmed-meshheading:9192769-Humans,
pubmed-meshheading:9192769-Macrophage Inflammatory Proteins,
pubmed-meshheading:9192769-Mice,
pubmed-meshheading:9192769-Molecular Sequence Data,
pubmed-meshheading:9192769-Organ Specificity,
pubmed-meshheading:9192769-Receptors, CCR1,
pubmed-meshheading:9192769-Receptors, Chemokine,
pubmed-meshheading:9192769-Receptors, Cytokine,
pubmed-meshheading:9192769-Recombinant Fusion Proteins,
pubmed-meshheading:9192769-Ribonucleases,
pubmed-meshheading:9192769-Saccharomyces cerevisiae Proteins,
pubmed-meshheading:9192769-Sequence Alignment,
pubmed-meshheading:9192769-Sequence Homology, Amino Acid,
pubmed-meshheading:9192769-Species Specificity,
pubmed-meshheading:9192769-Substrate Specificity,
pubmed-meshheading:9192769-T-Lymphocytes, Cytotoxic,
pubmed-meshheading:9192769-Transcription Factors
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pubmed:year |
1997
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pubmed:articleTitle |
Molecular cloning and characterization of a cDNA, CHEMR1, encoding a chemokine receptor with a homology to the human C-C chemokine receptor, CCR-4.
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pubmed:affiliation |
Department of Microbiology and Immunology, and Walther Oncology Center, Indiana University School of Medicine, Indianapolis 46202-5120, USA.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.
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