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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
1997-8-1
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pubmed:abstractText |
1. The influence of different inhibitors of cytochrome P450 mono-oxygenase on the endothelium-dependent and -independent hyperpolarization in the isolated rat main mesenteric artery was investigated. 2. Application of acetylcholine (ACh; 1 microM) for 10 min evoked an endothelium-dependent peak hyperpolarization of about 18 mV followed by a partial recovery to a level 7 mV more negative than the resting value (-50.2 +/- 0.5 mV). 3. Proadifen (30 microM) completely and reversibly inhibited the ACh-induced hyperpolarization. Conversely, the imidazole antimycotics clotrimazole (30 microM) and miconazole (100 microM) had less effect on the peak endothelium-dependent hyperpolarization. The suicide substrate inhibitors 17-octadecynoic acid (17-ODYA; 5 microM) and 1-aminobenzotriazole (1-ABT; 2 mM) did not significantly influence endothelium-dependent hyperpolarization. 4. The endothelium-independent hyperpolarization (16 mV) evoked by leveromakalim (300 nM) was completely inhibited by proadifen as well as by clotrimazole and miconazole but was not affected by 17-ODYA or 1-ABT. 5. These results do not support the view that the ACh-induced endothelium-dependent hyperpolarization in the rat mesenteric artery is mediated by cytochrome P450 mono-oxygenase metabolites. Proadifen and imidazole antimycotics impair the activation of ATP-regulated K+ channels in mesenteric artery cells, rendering non-specific inhibition of smooth muscle K+ channel activation an alternative explanation for the inhibitory influence of some (but not all) P450 inhibitors on endothelium-dependent hyperpolarization in this preparation.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/9192305-1376313,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9192305-1422587,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9192305-1510149,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9192305-1551193,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9192305-1706208,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9192305-1727681,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9192305-1908733,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9192305-2106399,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9192305-2366864,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9192305-2453240,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9192305-2545495,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9192305-2795490,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9192305-2851359,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9192305-2988418,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9192305-3074543,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9192305-6262771,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9192305-6297832,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9192305-6411899,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9192305-7510950,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9192305-7536941,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9192305-7562643,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9192305-7582531,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9192305-7712035,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9192305-7840155,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9192305-7889312,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9192305-8021834,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9192305-8422904,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9192305-8593700,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9192305-8646403,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9192305-8730760,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9192305-8818337,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9192305-8904643
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0022-3751
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
501 ( Pt 2)
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
331-41
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:9192305-Animals,
pubmed-meshheading:9192305-Arachidonic Acid,
pubmed-meshheading:9192305-Biological Factors,
pubmed-meshheading:9192305-Cyclooxygenase Inhibitors,
pubmed-meshheading:9192305-Cytochrome P-450 Enzyme System,
pubmed-meshheading:9192305-Electric Stimulation,
pubmed-meshheading:9192305-Electrophysiology,
pubmed-meshheading:9192305-Endothelium, Vascular,
pubmed-meshheading:9192305-Enzyme Inhibitors,
pubmed-meshheading:9192305-Indomethacin,
pubmed-meshheading:9192305-Kinetics,
pubmed-meshheading:9192305-Membrane Potentials,
pubmed-meshheading:9192305-Mesenteric Arteries,
pubmed-meshheading:9192305-Phospholipases A,
pubmed-meshheading:9192305-Quinacrine,
pubmed-meshheading:9192305-Rats,
pubmed-meshheading:9192305-Rats, Wistar
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pubmed:year |
1997
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pubmed:articleTitle |
Evidence against the involvement of cytochrome P450 metabolites in endothelium-dependent hyperpolarization of the rat main mesenteric artery.
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pubmed:affiliation |
Department of Physiology and Physiopathology, University of Gent, Belgium. Bert.Vanheel@rug.ac.be
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, Non-U.S. Gov't
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