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rdf:type |
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lifeskim:mentions |
umls-concept:C0010284,
umls-concept:C0030685,
umls-concept:C0178719,
umls-concept:C0206181,
umls-concept:C0242485,
umls-concept:C0391871,
umls-concept:C0439581,
umls-concept:C0521115,
umls-concept:C0596235,
umls-concept:C0680255,
umls-concept:C1283071,
umls-concept:C1547530,
umls-concept:C1707723,
umls-concept:C1963578
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pubmed:dateCreated |
1997-8-1
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pubmed:abstractText |
1. A technique has been developed to monitor neurotransmitter release simultaneously with intracellular Ca2+ concentration ([Ca2+]i) in single release boutons whose diameters range from 3 to 5 microns. 2. Using this technique, we have found a highly non-linear relationship between the rate of asynchronous release and [Ca2+]i. The Hill coefficient lies between 3 and 4. 3. The affinity (Kd) of the putative release-related Ca2+ receptor for asynchronous release was calculated to be in the range of 2-4 microM. 4. The same range of values of Hill coefficient and Kd were obtained when [Ca2+]i was elevated both by bath application of ionomycin and by repetitive stimulation at high frequency. 5. Our results show that the Ca2+ receptor(s) associated with asynchronous release exhibits high affinity for Ca2+.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/9192298-1331424,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9192298-1354503,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9192298-1432709,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9192298-1679119,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9192298-1683751,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9192298-2412607,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9192298-2418887,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9192298-2436546,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9192298-2795140,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9192298-283425,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9192298-2982166,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9192298-3838314,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9192298-4147099,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9192298-6055815,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9192298-6086856,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9192298-6106090,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9192298-6130509,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9192298-6261850,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9192298-6269044,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9192298-6805332,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9192298-7696493,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9192298-7790906,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9192298-7833845,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9192298-7954835,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9192298-7983538,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9192298-8101226,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9192298-8261139,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9192298-8520574,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9192298-8613732,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9192298-8652611,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9192298-8713455,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9192298-8747220,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9192298-8953257,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9192298-8985878
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0022-3751
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
501 ( Pt 2)
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
251-62
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:9192298-Animals,
pubmed-meshheading:9192298-Astacoidea,
pubmed-meshheading:9192298-Calcium,
pubmed-meshheading:9192298-Calibration,
pubmed-meshheading:9192298-Electric Stimulation,
pubmed-meshheading:9192298-Fluorescent Dyes,
pubmed-meshheading:9192298-Fura-2,
pubmed-meshheading:9192298-Image Processing, Computer-Assisted,
pubmed-meshheading:9192298-Kinetics,
pubmed-meshheading:9192298-Muscles,
pubmed-meshheading:9192298-Neurons,
pubmed-meshheading:9192298-Neurotransmitter Agents,
pubmed-meshheading:9192298-Synapses
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pubmed:year |
1997
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pubmed:articleTitle |
Simultaneous measurement of intracellular Ca2+ and asynchronous transmitter release from the same crayfish bouton.
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pubmed:affiliation |
Otto Loewi Centre for Cellular and Molecular Neurobiology, Department of Neurobiology, Hebrew University, Jerusalem, Israel.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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