Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:dateCreated |
1997-7-3
|
| pubmed:abstractText |
The epitopes recognized by 42 monoclonal antibodies directed against the human parainfluenza virus type 2 (hPIV-2) phosphoprotein (P) were mapped on the primary structure of the P protein by testing their reactivities with deletion mutants. By Western Immunoblotting with these monoclonal antibodies and P protein deletion mutants the region essential for P-P interactions was determined. The P protein region encompassing amino acids 211-248 was required for proper folding and oligomerization which is mediated by predicted coiled-coils in this region. The oligomer was shown to be a homotrimer by chemical cross-linking experiments.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jun
|
| pubmed:issn |
0022-1317
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
78 ( Pt 6)
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1303-8
|
| pubmed:dateRevised |
2004-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:9191922-Animals,
pubmed-meshheading:9191922-Antibodies, Monoclonal,
pubmed-meshheading:9191922-Blotting, Western,
pubmed-meshheading:9191922-COS Cells,
pubmed-meshheading:9191922-Epitope Mapping,
pubmed-meshheading:9191922-HeLa Cells,
pubmed-meshheading:9191922-Humans,
pubmed-meshheading:9191922-Parainfluenza Virus 2, Human,
pubmed-meshheading:9191922-Phosphoproteins,
pubmed-meshheading:9191922-Viral Proteins
|
| pubmed:year |
1997
|
| pubmed:articleTitle |
Human parainfluenza virus type 2 phosphoprotein: mapping of monoclonal antibody epitopes and location of the multimerization domain.
|
| pubmed:affiliation |
Department of Microbiology, Mie University School of Medicine, Japan. nishio@doc.medic.mie-u.ac.jp
|
| pubmed:publicationType |
Journal Article
|