Linked Life Data

9190895

Source:http://linkedlifedata.com/resource/pubmed/id/9190895

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
23
pubmed:dateCreated
1997-7-24
pubmed:abstractText
The AKT2 oncogene encodes a protein-serine/threonine kinase containing a pleckstrin homology domain characteristic of many signaling proteins. Recently, it was shown that AKT2 kinase activity can be induced by platelet-derived growth factor through phosphatidylinositol-3-OH kinase, suggesting that AKT2 may be an important signal mediator that contributes to the control of cell proliferation. We previously reported amplification and overexpression of AKT2 in human cancers. To investigate the transforming activity of AKT2, we used a retrovirus-based construct to express AKT2 in NIH3T3 cells. Overexpression of AKT2 was found to transform NIH3T3 cells, as determined by growth in soft agar and tumor formation in nude mice. The oncogenic activity of AKT2 was diminished by truncation of a 70-amino acid proline-rich region at the carboxyl-terminus. To facilitate the characterization of AKT2, we generated monoclonal and polyclonal antibodies against this protein. AKT2 was localized to the cytoplasm by cell fractionation experiments, immunocytochemistry, and immunofluorescence. Protein levels were more abundant in mitotic cells than in interphase cells. Western blot analysis of synchronized pancreatic cancer cells demonstrated that the expression level of AKT2 protein in mitotic cells is three to fivefold higher than in their interphase counterparts. A time-course study of phytohemagglutinin-stimulated lymphocytes revealed that AKT2 mRNA and AKT2 protein levels are highest 48-72 h after addition of mitogen, when cells are actively dividing. These data suggest that AKT2 could play a significant role in cell cycle progression and that the oncogenic activity of overexpressed AKT2 may be mediated by aberrant regulation of cellular proliferation.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0950-9232
pubmed:author
pubmed:issnType
Print
pubmed:day
12
pubmed:volume
14
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2793-801
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:9190895-3T3 Cells, pubmed-meshheading:9190895-Animals, pubmed-meshheading:9190895-Antibodies, Monoclonal, pubmed-meshheading:9190895-Cell Cycle, pubmed-meshheading:9190895-Cell Transformation, Neoplastic, pubmed-meshheading:9190895-Fibroblasts, pubmed-meshheading:9190895-Fluorescent Antibody Technique, Indirect, pubmed-meshheading:9190895-Gene Expression Regulation, Neoplastic, pubmed-meshheading:9190895-Humans, pubmed-meshheading:9190895-Interphase, pubmed-meshheading:9190895-Mice, pubmed-meshheading:9190895-Mice, Nude, pubmed-meshheading:9190895-Mitosis, pubmed-meshheading:9190895-Oncogene Proteins, pubmed-meshheading:9190895-Protein-Serine-Threonine Kinases, pubmed-meshheading:9190895-Proto-Oncogene Proteins, pubmed-meshheading:9190895-Proto-Oncogene Proteins c-akt, pubmed-meshheading:9190895-Subcellular Fractions, pubmed-meshheading:9190895-Transfection
pubmed:year
1997
pubmed:articleTitle
Transforming activity and mitosis-related expression of the AKT2 oncogene: evidence suggesting a link between cell cycle regulation and oncogenesis.
pubmed:affiliation
Department of Medical Oncology, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't