Linked Life Data

9188487

Source:http://linkedlifedata.com/resource/pubmed/id/9188487

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
25
pubmed:dateCreated
1997-7-21
pubmed:abstractText
A rat liver nuclear protein, unimolecular quadruplex telomere-binding protein 25, (uqTBP25) is described that binds tightly and specifically single-stranded and unimolecular tetraplex forms of the vertebrate telomeric DNA sequence 5'-d(TTAGGG)n-3'. A near homogeneous uqTBP25 was purified by ammonium sulfate precipitation, chromatographic separation from other DNA binding proteins, and three steps of column chromatography. SDS-polyacrylamide gel electrophoresis and Superdex copyright 200 gel filtration disclosed for uqTBP25 subunit and native Mr values of 25.4 +/- 0.5 and 25.0 kDa, respectively. Sequences of uqTBP25 tryptic peptides were closely homologous, but not identical, to heterogeneous nuclear ribonucleoprotein A1, heterogeneous nuclear ribonucleoprotein A2/B1, and single-stranded DNA-binding proteins UP1 and HDP-1. Complexes of uqTBP25 with single-stranded or unimolecular quadruplex 5'-d(TTAGGG)4-3', respectively, had dissociation constants, Kd, of 2.2 or 13.4 nM. Relative to d(TTAGGG)4, complexes with 5'-r(UUAGGG)4-3', blunt-ended duplex telomeric DNA, or quadruplex telomeric DNA had >10 to >250-fold higher Kd values. Single base alterations within the d(TTAGGG) repeat increased the Kd of complexes with uqTBP25 by 9-215-fold. Association with uqTBP25 protected d(TTAGGG)4 against nuclease digestion, suggesting a potential role for the protein in telomeric DNA transactions.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
20
pubmed:volume
272
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
15881-90
pubmed:dateRevised
2010-6-28
pubmed:meshHeading
pubmed-meshheading:9188487-Animals, pubmed-meshheading:9188487-Binding Sites, pubmed-meshheading:9188487-Chromatography, High Pressure Liquid, pubmed-meshheading:9188487-DNA, pubmed-meshheading:9188487-DNA, Single-Stranded, pubmed-meshheading:9188487-DNA-Binding Proteins, pubmed-meshheading:9188487-G-Quadruplexes, pubmed-meshheading:9188487-Heterogeneous-Nuclear Ribonucleoprotein Group A-B, pubmed-meshheading:9188487-Heterogeneous-Nuclear Ribonucleoproteins, pubmed-meshheading:9188487-Kinetics, pubmed-meshheading:9188487-Liver, pubmed-meshheading:9188487-Nucleic Acid Conformation, pubmed-meshheading:9188487-Peptide Mapping, pubmed-meshheading:9188487-Rats, pubmed-meshheading:9188487-Ribonucleoproteins, pubmed-meshheading:9188487-Sequence Homology, Amino Acid, pubmed-meshheading:9188487-Telomere
pubmed:year
1997
pubmed:articleTitle
Sequence-specific binding protein of single-stranded and unimolecular quadruplex telomeric DNA from rat hepatocytes.
pubmed:affiliation
Unit of Biochemistry, The Bruce Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, P. O. Box 9649, Haifa 31096, Israel.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't