Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1997-8-7
pubmed:databankReference
pubmed:abstractText
Spc1 in Schizosaccharomyces pombe is a member of the stress-activated protein kinase family, an evolutionary conserved subfamily of mitogen-activated protein kinases (MAPKs). Spc1 is activated by a MAPK kinase homologue, Wis1, and negatively regulated by Pyp1 and Pyp2 tyrosine phosphatases. Mutations in the spc1+ and wis1+ genes cause a G2 cell cycle delay that is exacerbated during stress. Herein, we describe two upstream regulators of the Wis1-Spc1 cascade. wik1+ (Wis1 kinase) was identified from its homology to budding yeast SSK2, which encodes a MAPKK kinase that regulates the HOG1 osmosensing pathway. Delta wik1 cells are impaired in stress-induced activation of Spc1 and show a G2 cell cycle delay and osmosensitive growth. Moreover, overproduction of a constitutively active form of Wik1 induces hyperactivation of Spc1 in wis1(+)-dependent manner, suggesting that Wik1 regulates Spc1 through activation of Wis1. A mutation of mcs4+ (mitotic catastrophe suppressor) was originally isolated as a suppressor of the mitotic catastrophe phenotype of a cdc2-3w wee1-50 double mutant. We have found that mcs4- cells are defective at activation of Spc1 in response to various forms of stress. Epistasis analysis has placed Mcs4-upstream of Wik1 in the Spc1 activation cascade. These results indicate that Mcs4 is part of a sensor system for multiple environmental signals that modulates the timing of entry into mitosis by regulating the Wik1-Wis1-Spc1 kinase cascade. Inactivation of the sensor system delays the onset of mitosis and rescues lethal premature mitosis in cdc2-3w wee1-50 cells.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/9188094-1330321, http://linkedlifedata.com/resource/pubmed/commentcorrection/9188094-1448087, http://linkedlifedata.com/resource/pubmed/commentcorrection/9188094-1464319, http://linkedlifedata.com/resource/pubmed/commentcorrection/9188094-14731678, http://linkedlifedata.com/resource/pubmed/commentcorrection/9188094-1479360, http://linkedlifedata.com/resource/pubmed/commentcorrection/9188094-1588914, http://linkedlifedata.com/resource/pubmed/commentcorrection/9188094-16068179, http://linkedlifedata.com/resource/pubmed/commentcorrection/9188094-1628833, http://linkedlifedata.com/resource/pubmed/commentcorrection/9188094-1706223, http://linkedlifedata.com/resource/pubmed/commentcorrection/9188094-1729597, http://linkedlifedata.com/resource/pubmed/commentcorrection/9188094-1756736, http://linkedlifedata.com/resource/pubmed/commentcorrection/9188094-1849659, http://linkedlifedata.com/resource/pubmed/commentcorrection/9188094-2004705, http://linkedlifedata.com/resource/pubmed/commentcorrection/9188094-2005825, http://linkedlifedata.com/resource/pubmed/commentcorrection/9188094-2046669, http://linkedlifedata.com/resource/pubmed/commentcorrection/9188094-2358444, http://linkedlifedata.com/resource/pubmed/commentcorrection/9188094-2474475, http://linkedlifedata.com/resource/pubmed/commentcorrection/9188094-3032459, http://linkedlifedata.com/resource/pubmed/commentcorrection/9188094-3241624, http://linkedlifedata.com/resource/pubmed/commentcorrection/9188094-3428262, http://linkedlifedata.com/resource/pubmed/commentcorrection/9188094-3442828, http://linkedlifedata.com/resource/pubmed/commentcorrection/9188094-3453113, http://linkedlifedata.com/resource/pubmed/commentcorrection/9188094-3955656, http://linkedlifedata.com/resource/pubmed/commentcorrection/9188094-7501024, http://linkedlifedata.com/resource/pubmed/commentcorrection/9188094-7523111, http://linkedlifedata.com/resource/pubmed/commentcorrection/9188094-7624781, http://linkedlifedata.com/resource/pubmed/commentcorrection/9188094-7657164, http://linkedlifedata.com/resource/pubmed/commentcorrection/9188094-7681220, http://linkedlifedata.com/resource/pubmed/commentcorrection/9188094-7681363, http://linkedlifedata.com/resource/pubmed/commentcorrection/9188094-7914033, http://linkedlifedata.com/resource/pubmed/commentcorrection/9188094-7923353, http://linkedlifedata.com/resource/pubmed/commentcorrection/9188094-7982971, http://linkedlifedata.com/resource/pubmed/commentcorrection/9188094-7997261, http://linkedlifedata.com/resource/pubmed/commentcorrection/9188094-8183345, http://linkedlifedata.com/resource/pubmed/commentcorrection/9188094-8211183, http://linkedlifedata.com/resource/pubmed/commentcorrection/9188094-8467814, http://linkedlifedata.com/resource/pubmed/commentcorrection/9188094-8515817, http://linkedlifedata.com/resource/pubmed/commentcorrection/9188094-8515818, http://linkedlifedata.com/resource/pubmed/commentcorrection/9188094-8557036, http://linkedlifedata.com/resource/pubmed/commentcorrection/9188094-8557037, http://linkedlifedata.com/resource/pubmed/commentcorrection/9188094-8557039, http://linkedlifedata.com/resource/pubmed/commentcorrection/9188094-8557102, http://linkedlifedata.com/resource/pubmed/commentcorrection/9188094-8649397, http://linkedlifedata.com/resource/pubmed/commentcorrection/9188094-8808622, http://linkedlifedata.com/resource/pubmed/commentcorrection/9188094-8824587, http://linkedlifedata.com/resource/pubmed/commentcorrection/9188094-8824588
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
1059-1524
pubmed:author
pubmed:issnType
Print
pubmed:volume
8
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
409-19
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
More...