Linked Life Data

9186012

Source:http://linkedlifedata.com/resource/pubmed/id/9186012

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1997-8-13
pubmed:abstractText
gamma-Tubulin is central to the nucleation of microtubule assembly in vivo. Although it is most obviously located at microtubule organizing centers, it is also found in soluble cytoplasmic complexes. Characterizing these complexes and identifying proteins that interact with gamma-tubulin in vivo will be necessary if gamma-tubulin function is to be understood fully. We have begun to investigate soluble complexes of gamma-tubulin in Aspergillus nidulans, the organism in which gamma-tubulin was discovered and in which a great deal of genetic and molecular genetic analysis of gamma-tubulin has been carried out. We find that approximately 32% of the gamma-tubulin in A. nidulans is soluble. Sucrose density gradients revealed that the soluble gamma-tubulin is in 8-20S complexes with little or no monomeric gamma-tubulin present. In the presence of 0.5 M KCl the average size of the complexes decreased and a peak was present between 4S and 11S. Cross-linking experiments with a zero-length cross-linker suggest that gamma-tubulin in isolated nuclei and in intact hyphae interacts physically with three proteins with molecular weights of approximately 105, 95, and 80 kDa.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0886-1544
pubmed:author
pubmed:issnType
Print
pubmed:volume
37
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
149-58
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1997
pubmed:articleTitle
Characterization of gamma-tubulin complexes in Aspergillus nidulans and detection of putative gamma-tubulin interacting proteins.
pubmed:affiliation
Laboratory of Medical Mycology, Nagoya University School of Medicine, Aichi, Japan.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't