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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
13
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pubmed:dateCreated |
1997-7-28
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pubmed:abstractText |
The restriction of herpes virus latency to mammalian sensory ganglia has led to a search for tissue-specific regulatory molecules in these neurons which alter viral gene expression. We have recently shown that the POU-domain transcriptional regulator Brn-3.0 is abundantly expressed in the adult trigeminal ganglion. To begin to examine the hypothesis that Brn-3.0 might participate in the regulation of the HSV life-cycle, we used Brn-3.0 POU-domain protein as an affinity matrix, and biochemically screened the entire HSV genome for sites of Brn-3.0 binding. This screen identified several sites of the form TA/TA A T N A N TA/T, which significantly do not include the previously identified HSV octamer sequences. All of the selected sites occur in the <25% of the HSV genome which has not been assigned to open reading frames, suggesting that these sites may be transcriptional regulatory elements recognized by Brn-3.0 or another homeobox factor with similar DNA binding properties. However, these sites do not interact with Brn-3.0 with sufficiently high affinity to directly mediate transcriptional activation by Brn-3.0 alone in transfection assays. The experiments described also provide an effective general method for exhaustive screening of large viral genomes or sub-genomic fragments of eukaryotic DNA for sites of interaction with specific transcription factors.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/9185568-1281152,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9185568-1381354,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9185568-1654947,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9185568-1848599,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9185568-1980658,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9185568-2155008,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9185568-2170153,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9185568-2434993,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9185568-2474674,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9185568-2571937,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9185568-2710122,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9185568-2739723,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9185568-2823252,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9185568-2830986,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9185568-2830987,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9185568-2839594,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9185568-2842768,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9185568-7623109,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9185568-7691107,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9185568-7904822,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9185568-7935477,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9185568-8129727,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9185568-8139923,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9185568-8248179,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9185568-8396817,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9185568-8559654,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9185568-8645597,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9185568-8876243,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9185568-8955272,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9185568-9111308,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9185568-9116190
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Glutathione Transferase,
http://linkedlifedata.com/resource/pubmed/chemical/Octamer Transcription Factor-2,
http://linkedlifedata.com/resource/pubmed/chemical/POU2F2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Pou2f2 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Pou4f1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factor Brn-3,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factor Brn-3A,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0305-1048
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
25
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2589-94
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:9185568-Animals,
pubmed-meshheading:9185568-Base Sequence,
pubmed-meshheading:9185568-Binding, Competitive,
pubmed-meshheading:9185568-Binding Sites,
pubmed-meshheading:9185568-DNA, Viral,
pubmed-meshheading:9185568-DNA-Binding Proteins,
pubmed-meshheading:9185568-Glutathione Transferase,
pubmed-meshheading:9185568-Herpesvirus 1, Human,
pubmed-meshheading:9185568-Humans,
pubmed-meshheading:9185568-Mice,
pubmed-meshheading:9185568-Octamer Transcription Factor-2,
pubmed-meshheading:9185568-Recombinant Fusion Proteins,
pubmed-meshheading:9185568-Simplexvirus,
pubmed-meshheading:9185568-Transcription Factor Brn-3,
pubmed-meshheading:9185568-Transcription Factor Brn-3A,
pubmed-meshheading:9185568-Transcription Factors
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pubmed:year |
1997
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pubmed:articleTitle |
The POU-domain factor Brn-3.0 recognizes characteristic sites in the herpes simplex virus genome.
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pubmed:affiliation |
Department of Psychiatry, University of California, San Diego, La Jolla, CA 92093-0603, USA. eturner@ucsd.edu
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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