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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
12
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pubmed:dateCreated |
1997-7-21
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pubmed:abstractText |
The intracellular mechanism(s) underlying the upregulation of the hepatic Na+/taurocholate cotransporting polypeptide (ntcp) by prolactin (PRL) are unknown. In this report, we demonstrate a time-dependent increase in nuclear translocation of phosphorylated liver Stat5 (a member of the ignal ransducers and ctivators of ranscription family) that correlated with suckling-induced increases in serum PRL levels. In electrophoretic mobility gel shift assays, nuclear Stat5 exhibited specific DNA-binding ability towards IFN-gamma-activated sequence (GAS)-like elements (GLEs; 5'TTC/A-PyNPu-G/TAA-3') located in the -937 to -904 bp region of the ntcp promoter. Transient cotransfections in HepG2 cells revealed that PRL inducibility (2.5-3-fold) required coexpression of the long form of the PRL receptor (PRLRL) and Stat5. Deletion analysis mapped the PRLinducible region to -1237 to -758 bp of the ntcp promoter. Linking this 0.5-kb region to a heterologous thymidine kinase (tk) promoter, or linking multimerized ntcp GLEs either upstream of the ntcp minimal promoter (-158 to +47 bp) or the heterologous promoter conferred dose-dependent PRL responsiveness. The short form of the PRL receptor failed to transactivate ntcp GLEs. These results indicate that PRL acts via the PRLRL to facilitate Stat5 binding to ntcp-GLEs and to transcriptionally regulate ntcp.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/9185514-14907713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9185514-1578373,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9185514-1662392,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9185514-173523,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9185514-175242,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9185514-1874500,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9185514-1891468,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9185514-1935820,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9185514-1961729,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9185514-1992473,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9185514-2036972,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9185514-2293022,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9185514-2554900,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9185514-271968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9185514-2832068,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9185514-7508935,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9185514-7514531,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9185514-7515493,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9185514-7519723,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9185514-7534288,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9185514-7537382,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9185514-7537736,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9185514-7744007,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9185514-7840192,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9185514-7843070,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9185514-7925280,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9185514-7945260,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9185514-8166278,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9185514-8188682,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9185514-8229790,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9185514-8392875,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9185514-8517919,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9185514-8530402,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9185514-8621467,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9185514-8625436,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9185514-8636096,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9185514-8662994,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9185514-8664546,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9185514-8670419,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9185514-8737372,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9185514-8776725,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9185514-9038875
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-gamma,
http://linkedlifedata.com/resource/pubmed/chemical/Milk Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Organic Anion Transporters...,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphotyrosine,
http://linkedlifedata.com/resource/pubmed/chemical/Prolactin,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Prolactin,
http://linkedlifedata.com/resource/pubmed/chemical/STAT5 Transcription Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium,
http://linkedlifedata.com/resource/pubmed/chemical/Symporters,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators,
http://linkedlifedata.com/resource/pubmed/chemical/sodium-bile acid cotransporter
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0021-9738
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
99
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2906-14
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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