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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1-2
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pubmed:dateCreated |
1997-7-3
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pubmed:abstractText |
This study shows the expression at the cell surface of human thymic epithelial cells (TEC) of a neurotensin (NT)-like immunoreactivity. NT radio-immunoassay (RIA) revealed that cultured human TEC contain +/-5 ng immunoreactive (ir) NT/10(6) cells, of which 5% is associated with plasma cell membranes. HPLC analysis of NT-ir present in human TEC showed a major peak of NT-ir corresponding to NT1-13. NT-ir was not detected in the supernatant of human TEC cultures. Using an affinity column prepared with a anti-MHC class I monoclonal antibody, NT-ir-related peptides were retained on the column and eluted together with MHC class I-related proteins. According to the elution time on HPLC of these peptides, they correspond to intact NT1-13, as well as to smaller fragments of NT1-13.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Histocompatibility Antigens Class I,
http://linkedlifedata.com/resource/pubmed/chemical/Neuropeptides,
http://linkedlifedata.com/resource/pubmed/chemical/Neurotensin,
http://linkedlifedata.com/resource/pubmed/chemical/neurotensin-like immunoreactivity
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0165-5728
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pubmed:author | |
pubmed:issnType |
Print
|
pubmed:volume |
76
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
161-6
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:9184646-Cell Membrane,
pubmed-meshheading:9184646-Cells, Cultured,
pubmed-meshheading:9184646-Child, Preschool,
pubmed-meshheading:9184646-Chromatography, Affinity,
pubmed-meshheading:9184646-Epithelium,
pubmed-meshheading:9184646-Histocompatibility Antigens Class I,
pubmed-meshheading:9184646-Humans,
pubmed-meshheading:9184646-Infant,
pubmed-meshheading:9184646-Neuropeptides,
pubmed-meshheading:9184646-Neurotensin,
pubmed-meshheading:9184646-Thymus Gland
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pubmed:year |
1997
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pubmed:articleTitle |
Identification of neurotensin-related peptides in human thymic epithelial cell membranes and relationship with major histocompatibility complex class I molecules.
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pubmed:affiliation |
INSERM Unité 339, Hôpital Saint-Antoine, Paris, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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