Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
|
| pubmed:dateCreated |
1997-6-30
|
| pubmed:abstractText |
The recently discovered human virus known as Kaposi's sarcoma-associated herpesvirus (KSHV) or human herpesvirus-8 (HHV-8) has been associated with body-cavity-based lymphomas in AIDS patients. It is most closely related to two other herpesviruses, the Epstein-Barr virus and herpesvirus saimiri, which are known to be associated with lymphomas in humans and nonhuman primates, respectively. To determine whether KSHV/HHV-8 is involved in the pathogenesis of mycosis fungoides (MF) and related disorders, we used a genomic PCR assay followed by confirmatory Southern blot analysis with a nested oligonucleotide probe to analyze cases for the presence of this virus. The specimens studied included fresh-frozen lesional tissues obtained from 16 patients with MF, seven with lymphomatoid papulosis, seven with primary cutaneous CD30+ large cell lymphoma of T-cell lineage, and five with Hodgkin's disease. Two T-cell tumor lines were also studied: MT4 (derived from a patient with adult T-cell leukemia/lymphoma) and Jurkat (derived from a patient with T-cell acute lymphoblastic leukemia). All cases were uniformly negative for KSHV/HHV-8, whereas Kaposi's sarcoma-positive controls and human beta-globin DNA integrity controls were appropriately positive. These findings provide strong evidence against a role for KSHV/HHV-8 in the pathogenesis of MF or associated lymphoproliferative disorders.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Beta-Globulins,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Neoplasm,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jun
|
| pubmed:issn |
0022-202X
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
108
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
920-2
|
| pubmed:dateRevised |
2007-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:9182822-Adult,
pubmed-meshheading:9182822-Base Sequence,
pubmed-meshheading:9182822-Beta-Globulins,
pubmed-meshheading:9182822-Blotting, Southern,
pubmed-meshheading:9182822-Cells, Cultured,
pubmed-meshheading:9182822-DNA,
pubmed-meshheading:9182822-DNA, Neoplasm,
pubmed-meshheading:9182822-DNA, Viral,
pubmed-meshheading:9182822-DNA Primers,
pubmed-meshheading:9182822-Herpesvirus 8, Human,
pubmed-meshheading:9182822-Hodgkin Disease,
pubmed-meshheading:9182822-Humans,
pubmed-meshheading:9182822-Jurkat Cells,
pubmed-meshheading:9182822-Lymphoma, Large B-Cell, Diffuse,
pubmed-meshheading:9182822-Mycosis Fungoides,
pubmed-meshheading:9182822-Polymerase Chain Reaction,
pubmed-meshheading:9182822-Skin,
pubmed-meshheading:9182822-Skin Diseases, Papulosquamous,
pubmed-meshheading:9182822-Skin Neoplasms,
pubmed-meshheading:9182822-Tumor Cells, Cultured
|
| pubmed:year |
1997
|
| pubmed:articleTitle |
No evidence of KSHV/HHV-8 in mycosis fungoides or associated disorders.
|
| pubmed:affiliation |
Department of Dermatology, Case Western Reserve University, Cleveland, Ohio, USA.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
|