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pubmed-article:9182678pubmed:abstractTextAdhesion of monocytes to the endothelium in lesion-prone areas is one of the earliest events in fatty streak formation leading to atherogenesis. The molecular basis of increased monocyte adhesion is not fully characterized. We have identified a novel vascular monocyte adhesion-associated protein, VMAP-1, that plays a role in adhesion of monocytes to activated endothelium. Originally selected for its ability to block binding of a mouse monocyte-like cell line (WEHI78/24) to cytokine- or LPS-stimulated cultured mouse endothelial cells in vitro, antiVMAP-1 mAb LM151 cross-reacts with rabbit endothelium and blocks binding of human monocytes to cultured rabbit aortic endothelial cells stimulated with minimally modified low density lipoprotein, thought to be a physiologically relevant atherogenic stimulus. Most importantly, LM151 prevents adhesion of normal monocytes and monocytoid cells to intact aortic endothelium from cholesterol-fed rabbits in an ex vivo assay. VMAP-1 is a 50-kD protein. Immunohistology of vessels reveals focal constitutive expression in aorta and other large vessels. VMAP-1 is thus a novel vascular adhesion-associated protein that appears to play a critical role in monocyte adhesion to aortic endothelial cells in atherogenesis in vivo.lld:pubmed
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pubmed-article:9182678pubmed:authorpubmed-author:ButcherE CEClld:pubmed
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pubmed-article:9182678pubmed:year1997lld:pubmed
pubmed-article:9182678pubmed:articleTitleNovel vascular molecule involved in monocyte adhesion to aortic endothelium in models of atherogenesis.lld:pubmed
pubmed-article:9182678pubmed:affiliationLaboratory of Immunology and Vascular Biology, Department of Pathology, Stanford University, Stanford, California 94305, USA.lld:pubmed
pubmed-article:9182678pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:9182678pubmed:publicationTypeResearch Support, U.S. Gov't, P.H.S.lld:pubmed
pubmed-article:9182678pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
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