Linked Life Data

9177721

Source:http://linkedlifedata.com/resource/pubmed/id/9177721

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1997-7-21
pubmed:abstractText
Cytochrome P450 2E1 catalyzes the metabolic activation of nitrosamines and haloalkenes to carcinogenic and cytotoxic derivatives; however, the regulation of this P450 isozyme is not well understood. Hydroxylation of p-nitrophenol to p-nitrocatechol has been used as a marker of CYP2E1 activity, but currently available methodologies are not sufficiently sensitive to allow measurements in small tissue samples or in tissues with low activity such as lung. We describe here a method for measuring p-nitrocatechol formation using HPLC with electrochemical detection which is rapid and specific. It has a level of sensitivity (pmol) sufficient to monitor CYP2E1 activities in incubations containing as little as 10 micrograms microsomal protein prepared from airway subcompartments of the lung, a tissue with low and varying CYP2E1 activities among different parts of the airways.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0003-2697
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
248
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
26-30
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1997
pubmed:articleTitle
Measurement of cytochrome P450 2E1 activity in rat tracheobronchial airways using high-performance liquid chromatography with electrochemical detection.
pubmed:affiliation
Department of Molecular Biosciences, School of Veterinary Medicine, University of California at Davis 95616, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.