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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
5-6
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pubmed:dateCreated |
1997-8-18
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pubmed:abstractText |
Biosensors for the determination of sugars were constructed using oligosaccharide dehydrogenase (ODH) and soluble phenazine methosulfate (PMS) or an osmium-based three-dimensional redox hydrogel. In the latter case the enzyme and poly(1-vinylimidazole) complexed with osmium (4,4'-dimethylbpy)2Cl were cross-linked with poly(ethylene glycol) diglycidyl ether. Both electrode configurations showed similar sensitivities for glucose in the range between 8 and 21 muAmM-1 cm-2. The responses for 10 mono and oligosaccharides were studied. There was no response for fructose. In the concentration range 0.1-20 mM the relative sensitivities were determined for arabinose (96%), xylose (3%), mannose (50%), galactose (11%), glucose (100%), maltose (24%), lactose (12%), cellobiose (34%) and maltotriose (10%).
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Carbohydrate Dehydrogenases,
http://linkedlifedata.com/resource/pubmed/chemical/Glucose,
http://linkedlifedata.com/resource/pubmed/chemical/Methylphenazonium Methosulfate,
http://linkedlifedata.com/resource/pubmed/chemical/Oligosaccharides,
http://linkedlifedata.com/resource/pubmed/chemical/Osmium,
http://linkedlifedata.com/resource/pubmed/chemical/Polymers
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pubmed:status |
MEDLINE
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pubmed:issn |
0952-3499
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
9
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
480-4
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:9174927-Biosensing Techniques,
pubmed-meshheading:9174927-Calibration,
pubmed-meshheading:9174927-Carbohydrate Dehydrogenases,
pubmed-meshheading:9174927-Electrodes,
pubmed-meshheading:9174927-Glucose,
pubmed-meshheading:9174927-Methylphenazonium Methosulfate,
pubmed-meshheading:9174927-Molecular Weight,
pubmed-meshheading:9174927-Oligosaccharides,
pubmed-meshheading:9174927-Osmium,
pubmed-meshheading:9174927-Polymers,
pubmed-meshheading:9174927-Sensitivity and Specificity
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pubmed:articleTitle |
Preliminary investigations of an amperometric oligosaccharide dehydrogenase-based electrode for the detection of glucose and some other low molecular weight saccharides.
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pubmed:affiliation |
Enzyme Chemistry Laboratory, Institute of Biochemistry, Vilnius, Lithuania.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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