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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:dateCreated |
1997-8-20
|
| pubmed:abstractText |
Retroviral particles associated with reverse transcriptase (RT) activity in cell-cultures from MS patients have been reported by different groups. Cell-cultures have been used for the study and characterization of the corresponding retroviral genome which we have shown is related to ERV9 in the pol region. Previously unpublished details of a study with monocyte cultures are presented together with observations on leptomeningeal and choroid-plexus cultures. The generation of self-transformed cultures after inhibition of interferon, followed by the loss of retroviral expression and recurrent apoptosis, is analyzed. Retroviral particles with RT-activity are produced in monocyte cultures with an apparent correlation with MS disease activity. However, though leptomeningeal and choroid plexus cells from MS can be passaged for a limited period, their evolution in vitro is not compatible with stable retroviral expression. These culture limitations greatly hampered progress on the elucidation of the retroviral genome sequence.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:issn |
0065-1427
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
169
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
22-31
|
| pubmed:dateRevised |
2007-7-23
|
| pubmed:meshHeading |
pubmed-meshheading:9174637-Adult,
pubmed-meshheading:9174637-Aged,
pubmed-meshheading:9174637-Animals,
pubmed-meshheading:9174637-Cell Division,
pubmed-meshheading:9174637-Cell Line, Transformed,
pubmed-meshheading:9174637-Cell Transformation, Neoplastic,
pubmed-meshheading:9174637-Cell Transformation, Viral,
pubmed-meshheading:9174637-Cells, Cultured,
pubmed-meshheading:9174637-Choroid Plexus,
pubmed-meshheading:9174637-Female,
pubmed-meshheading:9174637-Gene Expression Regulation, Viral,
pubmed-meshheading:9174637-Genes, pol,
pubmed-meshheading:9174637-Humans,
pubmed-meshheading:9174637-Male,
pubmed-meshheading:9174637-Meninges,
pubmed-meshheading:9174637-Mice,
pubmed-meshheading:9174637-Mice, Nude,
pubmed-meshheading:9174637-Middle Aged,
pubmed-meshheading:9174637-Monocytes,
pubmed-meshheading:9174637-Multiple Sclerosis,
pubmed-meshheading:9174637-RNA-Directed DNA Polymerase,
pubmed-meshheading:9174637-Retroviridae Infections
|
| pubmed:year |
1997
|
| pubmed:articleTitle |
Cell cultures and associated retroviruses in multiple sclerosis. Collaborative Research Group on MS.
|
| pubmed:affiliation |
BioMérieux S.A., UMR 103 CNRS-bioMérieux, Lyon, France.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|