Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
|
| pubmed:dateCreated |
1997-6-30
|
| pubmed:abstractText |
We have investigated the role of specific components of the thymic stroma during development of CD4-8-T cell precursors by separating and reaggregating precursor subsets with individual or combinations of stromal cells. We show that while the development of CD25+ 44+ precursors is dependent upon a combination of major histocompatibility complex (MHC) class II+ thymic epithelial cells and fibroblasts, their direct descendants, CD25+ 44- precursors, develop to the CD4+ 8+ stage in the presence of MHC class II+ thymic epithelial cells alone. Thus, CD25+ 44+ precursors are the last developmental stage to be dependent upon fibroblast support. In addition, while metabolically inactive, 1-ethyl-3-(3'-dimethylaminopropyl) carbodiimide (ECDI)-treated fibroblasts retain the ability to promote T cell development, prior treatment with hyaluronidase abrogates this effect, suggesting that fibroblast-associated extracellular matrix components are the key elements involved. In support of this, we show that fibroblasts are located in cortical regions of the thymus where T cell precursors are known to reside, and that these fibroblasts are associated with an extensive extracellular matrix not found on thymic epithelial cells. Finally, antibodies to alpha 4 integrin and CD44 interfere with the efficiency with which CD4+ 8+ cells are generated from CD25+ 44+ precursors in reaggregate cultures and also reduce the binding of the latter to 3T3 fibroblasts, suggesting these molecules play a role in bringing T cell precursors into contact with fibroblast-associated extracellular matrix.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD4,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD44,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD8,
http://linkedlifedata.com/resource/pubmed/chemical/Ethyldimethylaminopropyl...,
http://linkedlifedata.com/resource/pubmed/chemical/Extracellular Matrix Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Hyaluronoglucosaminidase,
http://linkedlifedata.com/resource/pubmed/chemical/Integrin alpha4,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Interleukin-2
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
May
|
| pubmed:issn |
0014-2980
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
27
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1200-6
|
| pubmed:dateRevised |
2009-9-29
|
| pubmed:meshHeading |
pubmed-meshheading:9174611-3T3 Cells,
pubmed-meshheading:9174611-Animals,
pubmed-meshheading:9174611-Animals, Newborn,
pubmed-meshheading:9174611-Antibodies, Monoclonal,
pubmed-meshheading:9174611-Antigens, CD,
pubmed-meshheading:9174611-Antigens, CD4,
pubmed-meshheading:9174611-Antigens, CD44,
pubmed-meshheading:9174611-Antigens, CD8,
pubmed-meshheading:9174611-Cell Aggregation,
pubmed-meshheading:9174611-Cell Communication,
pubmed-meshheading:9174611-Cell Differentiation,
pubmed-meshheading:9174611-Ethyldimethylaminopropyl Carbodiimide,
pubmed-meshheading:9174611-Extracellular Matrix Proteins,
pubmed-meshheading:9174611-Fibroblasts,
pubmed-meshheading:9174611-Hematopoietic Stem Cells,
pubmed-meshheading:9174611-Hyaluronoglucosaminidase,
pubmed-meshheading:9174611-Integrin alpha4,
pubmed-meshheading:9174611-Mice,
pubmed-meshheading:9174611-Mice, Inbred BALB C,
pubmed-meshheading:9174611-Rabbits,
pubmed-meshheading:9174611-Receptors, Interleukin-2,
pubmed-meshheading:9174611-Stromal Cells,
pubmed-meshheading:9174611-T-Lymphocyte Subsets,
pubmed-meshheading:9174611-Thymus Gland
|
| pubmed:year |
1997
|
| pubmed:articleTitle |
Fibroblast dependency during early thymocyte development maps to the CD25+ CD44+ stage and involves interactions with fibroblast matrix molecules.
|
| pubmed:affiliation |
Department of Anatomy/Centre for Clinical Research in Immunology and Signalling, Medical School, University of Birmingham, GB. andersog@novell2.bham.ac.uk
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|