Linked Life Data

9169851

Source:http://linkedlifedata.com/resource/pubmed/id/9169851

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
1997-6-27
pubmed:databankReference
pubmed:abstractText
The first somatic single cells of carrot hypocotyl explants having the competence to form embryos in the presence of 2,4-dichlorophenoxyacetic acid (2,4-D) were identified using semi-automatic cell tracking. These competent cells are present as a small subpopulation of enlarged and vacuolated cells derived from cytoplasm-rich and rapidly proliferating non-embryogenic cells that originate from the provascular elements of the hypocotyl. A search for marker genes to monitor the transition of somatic into competent and embryogenic cells in established suspension cell cultures resulted in the identification of a gene transiently expressed in a small subpopulation of the same enlarged single cells that are formed during the initiation of the embryogenic cultures from hypocotyl explants. The predicted amino acid sequence and in vitro kinase assays show that this gene encodes a leucine-rich repeat containing receptor-like kinase protein, designated Somatic Embryogenesis Receptor-like Kinase (SERK). Somatic embryos formed from cells expressing a SERK promoter-luciferase reporter gene. During somatic embryogenesis, SERK expression ceased after the globular stage. In plants, SERK mRNA could only be detected transiently in the zygotic embryo up to the early globular stage but not in unpollinated flowers nor in any other plant tissue. These results suggest that somatic cells competent to form embryos and early globular somatic embryos share a highly specific signal transduction chain with the zygotic embryo from shortly after fertilization to the early globular embryo.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0950-1991
pubmed:author
pubmed:issnType
Print
pubmed:volume
124
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2049-62
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:9169851-2,4-Dichlorophenoxyacetic Acid, pubmed-meshheading:9169851-Amino Acid Sequence, pubmed-meshheading:9169851-Base Sequence, pubmed-meshheading:9169851-Cells, Cultured, pubmed-meshheading:9169851-Cloning, Molecular, pubmed-meshheading:9169851-DNA, Complementary, pubmed-meshheading:9169851-Daucus carota, pubmed-meshheading:9169851-Gene Dosage, pubmed-meshheading:9169851-Gene Expression Regulation, Plant, pubmed-meshheading:9169851-Genes, Plant, pubmed-meshheading:9169851-Herbicides, pubmed-meshheading:9169851-Hypocotyl, pubmed-meshheading:9169851-Leucine, pubmed-meshheading:9169851-Molecular Sequence Data, pubmed-meshheading:9169851-Plant Proteins, pubmed-meshheading:9169851-Protein Kinases, pubmed-meshheading:9169851-RNA, Messenger, pubmed-meshheading:9169851-RNA, Plant, pubmed-meshheading:9169851-Recombinant Fusion Proteins, pubmed-meshheading:9169851-Sequence Analysis, DNA, pubmed-meshheading:9169851-Sequence Homology, Amino Acid
pubmed:year
1997
pubmed:articleTitle
A leucine-rich repeat containing receptor-like kinase marks somatic plant cells competent to form embryos.
pubmed:affiliation
Department of Molecular Biology, Wageningen Agricultural Univeristy, the Netherlands.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't