Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
23
|
| pubmed:dateCreated |
1997-6-26
|
| pubmed:abstractText |
Thymus and activation-regulated chemokine (TARC) is a recently identified CC chemokine that is expressed constitutively in thymus and transiently in stimulated peripheral blood mononuclear cells. TARC functions as a selective chemoattractant for T cells that express a class of receptors binding TARC with high affinity and specificity. To identify the receptor for TARC, we produced TARC as a fusion protein with secreted alkaline phosphatase (SEAP) and used it for specific binding. By stably transfecting five orphan receptors and five known CC chemokine receptors (CCR1 to -5) into K562 cells, we found that TARC-SEAP bound selectively to cells expressing CCR4. TARC-SEAP also bound to K562 cells stably expressing CCR4 with a high affinity (Kd = 0.5 nM). Only TARC and not five other CC chemokines (MCP-1 (monocyte chemoattractant protein-1), RANTES (regulated upon activation, normal T cells expressed and secreted), MIP-1alpha (macrophage inflammatory protein-1alpha), MIP-1beta, and LARC (liver and activation-regulated chemokine)) competed with TARC-SEAP for binding to CCR4. TARC but not RANTES or MIP-1alpha induced migration and calcium mobilization in 293/EBNA-1 cells stably expressing CCR4. K562 cells stably expressing CCR4 also responded to TARC in a calcium mobilization assay. Northern blot analysis revealed that CCR4 mRNA was expressed strongly in human T cell lines and peripheral blood T cells but not in B cells, natural killer cells, monocytes, or granulocytes. Taken together, TARC is a specific functional ligand for CCR4, and CCR4 is the specific receptor for TARC selectively expressed on T cells.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Alkaline Phosphatase,
http://linkedlifedata.com/resource/pubmed/chemical/CCL17 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/CCR4 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Chemokine CCL17,
http://linkedlifedata.com/resource/pubmed/chemical/Chemokine CCL5,
http://linkedlifedata.com/resource/pubmed/chemical/Chemokines,
http://linkedlifedata.com/resource/pubmed/chemical/Chemokines, CC,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, CCR4,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Chemokine,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cytokine,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jun
|
| pubmed:issn |
0021-9258
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
6
|
| pubmed:volume |
272
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
15036-42
|
| pubmed:dateRevised |
2007-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:9169480-Alkaline Phosphatase,
pubmed-meshheading:9169480-B-Lymphocytes,
pubmed-meshheading:9169480-Binding, Competitive,
pubmed-meshheading:9169480-Calcium,
pubmed-meshheading:9169480-Cell Line,
pubmed-meshheading:9169480-Chemokine CCL17,
pubmed-meshheading:9169480-Chemokine CCL5,
pubmed-meshheading:9169480-Chemokines,
pubmed-meshheading:9169480-Chemokines, CC,
pubmed-meshheading:9169480-Chemotaxis,
pubmed-meshheading:9169480-Humans,
pubmed-meshheading:9169480-Kinetics,
pubmed-meshheading:9169480-Polymerase Chain Reaction,
pubmed-meshheading:9169480-Receptors, CCR4,
pubmed-meshheading:9169480-Receptors, Chemokine,
pubmed-meshheading:9169480-Receptors, Cytokine,
pubmed-meshheading:9169480-Recombinant Fusion Proteins,
pubmed-meshheading:9169480-T-Lymphocytes,
pubmed-meshheading:9169480-Transfection,
pubmed-meshheading:9169480-Tumor Cells, Cultured
|
| pubmed:year |
1997
|
| pubmed:articleTitle |
The T cell-directed CC chemokine TARC is a highly specific biological ligand for CC chemokine receptor 4.
|
| pubmed:affiliation |
Shionogi Institute for Medical Science, 2-5-1 Mishima, Settsu-shi, Osaka 566, Japan. toshio.imai@shionogi.co.jp
|
| pubmed:publicationType |
Journal Article,
Comparative Study
|