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rdf:type |
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lifeskim:mentions |
umls-concept:C0017262,
umls-concept:C0017337,
umls-concept:C0019704,
umls-concept:C0021760,
umls-concept:C0033684,
umls-concept:C0035668,
umls-concept:C0040648,
umls-concept:C0185117,
umls-concept:C0205263,
umls-concept:C0209548,
umls-concept:C0330390,
umls-concept:C0961954,
umls-concept:C1100939,
umls-concept:C1167622,
umls-concept:C2242140,
umls-concept:C2911684
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pubmed:issue |
23
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pubmed:dateCreated |
1997-6-26
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pubmed:abstractText |
Human immunodeficiency virus type 1 (HIV-1) infection is associated with severe psoriasis, B cell lymphoma, and Kaposi's sarcoma. A deregulated production of interleukin-6 (IL6) has been implicated in the pathogenesis of these diseases. The molecular mechanisms underlying the abnormal IL6 secretion of HIV-1-infected cells may include transactivation of the IL6 gene by HIV-1. Here we report the molecular mechanisms of Tat activity on the expression of the IL6 gene. By using 5' deletion mutants of pIL6Pr-CAT and using IL6:HIV-1-LTR hybrid constructs where discrete regions of the IL6 promoter replaced the TAR sequence in HIV-1 LTR, we identified a short sequence of the 5'-untranslated region of the IL6 mRNA that is required for Tat to trans-activate the IL6 promoter. This sequence acquires a stem-loop structure and includes a UCU sequence that binds to Tat and is necessary for full trans-activation. In addition, we provide the evidence that Tat can function by enhancing the CAAT enhancer-binding protein (C/EBP) DNA binding activity and is able to complex with in vitro translated C/EBPbeta, which is a major mediator of IL6 promoter function. By using the yeast two-hybrid system and immunoprecipitation, we observed that the interaction of Tat with C/EBP proteins also occurred in vivo. The data are consistent with the possibility that Tat may function on heterologous genes by interacting with RNA structures possibly present in a large number of cellular and viral genes. In addition, Tat may function by protein-protein interactions, leading to the generation of heterodimers with specific transcription factors.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/CCAAT-Enhancer-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Chloramphenicol O-Acetyltransferase,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Gene Products, tat,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-6,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Oligonucleotide Probes,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/tat Gene Products, Human...
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
6
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pubmed:volume |
272
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
14883-92
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:9169458-CCAAT-Enhancer-Binding Proteins,
pubmed-meshheading:9169458-Chloramphenicol O-Acetyltransferase,
pubmed-meshheading:9169458-Cloning, Molecular,
pubmed-meshheading:9169458-DNA Primers,
pubmed-meshheading:9169458-DNA-Binding Proteins,
pubmed-meshheading:9169458-Gene Products, tat,
pubmed-meshheading:9169458-Genes, tat,
pubmed-meshheading:9169458-HIV-1,
pubmed-meshheading:9169458-HeLa Cells,
pubmed-meshheading:9169458-Humans,
pubmed-meshheading:9169458-Interleukin-6,
pubmed-meshheading:9169458-Mutagenesis, Site-Directed,
pubmed-meshheading:9169458-Nuclear Proteins,
pubmed-meshheading:9169458-Oligonucleotide Probes,
pubmed-meshheading:9169458-Plasmids,
pubmed-meshheading:9169458-Promoter Regions, Genetic,
pubmed-meshheading:9169458-RNA, Messenger,
pubmed-meshheading:9169458-Recombinant Fusion Proteins,
pubmed-meshheading:9169458-Sequence Deletion,
pubmed-meshheading:9169458-Transcription, Genetic,
pubmed-meshheading:9169458-Transcription Factors,
pubmed-meshheading:9169458-Transfection,
pubmed-meshheading:9169458-tat Gene Products, Human Immunodeficiency Virus
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pubmed:year |
1997
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pubmed:articleTitle |
HIV-1 Tat induces the expression of the interleukin-6 (IL6) gene by binding to the IL6 leader RNA and by interacting with CAAT enhancer-binding protein beta (NF-IL6) transcription factors.
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pubmed:affiliation |
Department of Clinical and Experimental Medicine, Medical School, University of Reggio Calabria, 88100 Catanzaro, Italy.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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