Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
23
pubmed:dateCreated
1997-6-26
pubmed:databankReference
pubmed:abstractText
The neurotransmitter serotonin mediates a wide variety of peripheral and central physiological effects through the binding to multiple receptor subtypes (Wilkinson, L. O., and Dourish, C. T. (1991) in Serotonin Receptor Subtypes: Basic and Clinical Aspects (Peroutka, S. J., ed) Vol. 15, pp.147-210, Wiley-Liss, New York). Among them, serotonin 5-HT2A receptors are known to activate the phospholipase C-beta second messenger pathway (Peroutka, S. J. (1995) Trends Neurosci. 18, 68-69). We identified and localized in rat skeletal muscle myoblasts a functional serotonin 5-HT2A receptor. This receptor was detected on the plasma membrane, in myoblasts, and at the level of T-tubules in contracting myotubes. Binding of serotonin to its receptor increases the expression of genes involved in myogenic differentiation. Unexpectedly, the 5-HT2A receptor is able to activate another signaling pathway; it triggers a rapid and transient tyrosine phosphorylation of Jak2 kinase in response to serotonin. Jak2 auto-phosphorylation is followed by the tyrosine phosphorylation of STAT3 (signal transducers and activators of transcription) and its translocation into the nucleus. We also find that the 5-HT2A receptor and STAT3 co-precipitate with Jak2, indicating that they are physically associated. We conclude that the serotonin 5-HT2A receptor identified in skeletal muscle myoblasts is able to activate the intracellular phosphorylation pathway used by cytokines. The presence of serotonin receptors in T-tubules suggests a role for serotonin in excitation-contraction coupling and (or) an effect in skeletal muscle fiber repairing.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Glucose Transporter Type 1, http://linkedlifedata.com/resource/pubmed/chemical/Glucose Transporter Type 2, http://linkedlifedata.com/resource/pubmed/chemical/Jak2 protein, rat, http://linkedlifedata.com/resource/pubmed/chemical/Janus Kinase 2, http://linkedlifedata.com/resource/pubmed/chemical/Monosaccharide Transport Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Myog protein, rat, http://linkedlifedata.com/resource/pubmed/chemical/Myogenin, http://linkedlifedata.com/resource/pubmed/chemical/Protein-Tyrosine Kinases, http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Receptor, Serotonin, 5-HT2A, http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Serotonin, http://linkedlifedata.com/resource/pubmed/chemical/STAT3 Transcription Factor, http://linkedlifedata.com/resource/pubmed/chemical/Serotonin, http://linkedlifedata.com/resource/pubmed/chemical/Stat3 protein, rat, http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
6
pubmed:volume
272
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
14825-9
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:9169451-Animals, pubmed-meshheading:9169451-Base Sequence, pubmed-meshheading:9169451-Cell Membrane, pubmed-meshheading:9169451-Cells, Cultured, pubmed-meshheading:9169451-DNA-Binding Proteins, pubmed-meshheading:9169451-Fetus, pubmed-meshheading:9169451-Glucose Transporter Type 1, pubmed-meshheading:9169451-Glucose Transporter Type 2, pubmed-meshheading:9169451-Janus Kinase 2, pubmed-meshheading:9169451-Molecular Sequence Data, pubmed-meshheading:9169451-Monosaccharide Transport Proteins, pubmed-meshheading:9169451-Muscle, Skeletal, pubmed-meshheading:9169451-Myogenin, pubmed-meshheading:9169451-Phosphorylation, pubmed-meshheading:9169451-Polymerase Chain Reaction, pubmed-meshheading:9169451-Protein-Tyrosine Kinases, pubmed-meshheading:9169451-Proto-Oncogene Proteins, pubmed-meshheading:9169451-Rats, pubmed-meshheading:9169451-Receptor, Serotonin, 5-HT2A, pubmed-meshheading:9169451-Receptors, Serotonin, pubmed-meshheading:9169451-STAT3 Transcription Factor, pubmed-meshheading:9169451-Serotonin, pubmed-meshheading:9169451-Signal Transduction, pubmed-meshheading:9169451-Trans-Activators, pubmed-meshheading:9169451-Transcription, Genetic
pubmed:year
1997
pubmed:articleTitle
Identification and localization of a skeletal muscle secrotonin 5-HT2A receptor coupled to the Jak/STAT pathway.
pubmed:affiliation
Centre de Recherches sur l'Endocrinologie Moléculaire et le Développement, CNRS, UPR 1511, 9, rue Jules Hetzel, 92 190 Meudon, France. gdeniau@infobiogen.fr
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't