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rdf:type |
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lifeskim:mentions |
umls-concept:C0017262,
umls-concept:C0017757,
umls-concept:C0025914,
umls-concept:C0026809,
umls-concept:C0185117,
umls-concept:C0205245,
umls-concept:C0600399,
umls-concept:C0679058,
umls-concept:C1547699,
umls-concept:C2700640,
umls-concept:C2911684
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pubmed:issue |
3
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pubmed:dateCreated |
1997-7-1
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pubmed:databankReference |
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pubmed:abstractText |
The X-chromosomal gene glucose-6-phosphate dehydrogenase (G6pd) is known to be expressed in most cell types of mammalian species. In the mouse, we have detected a novel gene, designated G6pd-2, encoding a G6PD isoenzyme. G6pd-2 does not contain introns and appears to represent a retroposed gene. This gene is uniquely transcribed in postmeiotic spermatogenic cells in which the X-encoded G6pd gene is not transcribed. Expression of the G6pd-2 sequence in a bacterial system showed that the encoded product is an active enzyme. Zymogramic analysis demonstrated that recombinant G6PD-2, but not recombinant G6PD-1 (the X-chromosome-encoded G6PD), formed tetramers under reducing conditions. Under the same conditions, G6PD tetramers were also found in extracts of spermatids and spermatozoa, indicating the presence of G6pd-2-encoded isoenzyme in these cell types. G6pd-2 is one of the very few known expressed retroposons encoding a functional protein, and the presence of this gene is probably related to X chromosome inactivation during spermatogenesis.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0888-7543
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
41
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
350-9
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:9169132-Amino Acid Sequence,
pubmed-meshheading:9169132-Animals,
pubmed-meshheading:9169132-Base Sequence,
pubmed-meshheading:9169132-DNA,
pubmed-meshheading:9169132-DNA Primers,
pubmed-meshheading:9169132-Dosage Compensation, Genetic,
pubmed-meshheading:9169132-Gene Expression,
pubmed-meshheading:9169132-Glucosephosphate Dehydrogenase,
pubmed-meshheading:9169132-Isoenzymes,
pubmed-meshheading:9169132-Male,
pubmed-meshheading:9169132-Mice,
pubmed-meshheading:9169132-Molecular Sequence Data,
pubmed-meshheading:9169132-Phylogeny,
pubmed-meshheading:9169132-RNA, Messenger,
pubmed-meshheading:9169132-Rats,
pubmed-meshheading:9169132-Retroelements,
pubmed-meshheading:9169132-Sequence Homology, Amino Acid,
pubmed-meshheading:9169132-Sequence Homology, Nucleic Acid,
pubmed-meshheading:9169132-Spermatogenesis,
pubmed-meshheading:9169132-Testis,
pubmed-meshheading:9169132-Tissue Distribution,
pubmed-meshheading:9169132-X Chromosome
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pubmed:year |
1997
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pubmed:articleTitle |
Testis-specific expression of a functional retroposon encoding glucose-6-phosphate dehydrogenase in the mouse.
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pubmed:affiliation |
Department of Endocrinology and Reproduction, Faculty of Medicine and Health Sciences, Erasmus University, Rotterdam, The Netherlands.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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