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rdf:type |
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lifeskim:mentions |
umls-concept:C0009015,
umls-concept:C0017337,
umls-concept:C0021469,
umls-concept:C0024370,
umls-concept:C0162801,
umls-concept:C0242709,
umls-concept:C0439861,
umls-concept:C0678594,
umls-concept:C0679058,
umls-concept:C1514562,
umls-concept:C1547699,
umls-concept:C1880389,
umls-concept:C1883204,
umls-concept:C1883221,
umls-concept:C2348205,
umls-concept:C2700640
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pubmed:issue |
2
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pubmed:dateCreated |
1997-6-16
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pubmed:databankReference |
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pubmed:abstractText |
The nucleotide sequence has been determined for zooA, a gene encoding the bacteriocin-like inhibitory substance zoocin A in Streptococcus zooepidemicus strain 4881. The zooA gene product corresponds to the 285-amino acid (aa) zoocin A pre-peptide from which a leader sequence is cleaved to form the 262-aa biologically active molecule of estimated molecular mass 27,877 Da. Expression of zooA in a Gram-negative host was shown by the extracellular release from Escherichia coli, containing cloned zooA, of a biologically active peptide having an identical range of anti-bacterial activity to that of zoocin A, purified from S. zooepidemicus strain 4881. Data base searches revealed sequences having homologies with known muralytic proteins produced by both Gram-positive and Gram-negative bacteria and indicate a 'mix and match' blending of domain-type structures, the C-terminal putative receptor-recognition region of the molecule being joined by a threonine-proline-rich linker to an N-terminal putative catalytic region having homology with several known endopeptidases, including lysostaphin.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0378-1119
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
21
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pubmed:volume |
189
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
255-61
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:9168135-Amino Acid Sequence,
pubmed-meshheading:9168135-Anti-Bacterial Agents,
pubmed-meshheading:9168135-Bacterial Proteins,
pubmed-meshheading:9168135-Bacteriocins,
pubmed-meshheading:9168135-Base Sequence,
pubmed-meshheading:9168135-Cloning, Molecular,
pubmed-meshheading:9168135-Escherichia coli,
pubmed-meshheading:9168135-Genes, Bacterial,
pubmed-meshheading:9168135-Lysostaphin,
pubmed-meshheading:9168135-Molecular Sequence Data,
pubmed-meshheading:9168135-Peptides,
pubmed-meshheading:9168135-Protein Structure, Tertiary,
pubmed-meshheading:9168135-Sequence Analysis, DNA,
pubmed-meshheading:9168135-Sequence Homology, Amino Acid,
pubmed-meshheading:9168135-Streptococcus equi
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pubmed:year |
1997
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pubmed:articleTitle |
Cloning and sequence analysis of zooA, a Streptococcus zooepidemicus gene encoding a bacteriocin-like inhibitory substance having a domain structure similar to that of lysostaphin.
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pubmed:affiliation |
Department of Microbiology, University of Otago, Dunedin, New Zealand.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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