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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1997-8-4
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pubmed:abstractText |
Homologous recombination in Escherichia coli is initiated by the RecBCD enzyme, and is stimulated by DNA elements known as Chi (chi) sites. The RecBCD enzyme is both a helicase and a nuclease. Recognition of chi causes both attenuation of the 3'-->5' exonuclease activity of the RecBCD enzyme, and activation of an exonuclease activity with 5'-->3' polarity, while leaving the helicase activity unaffected. A variety of evidence suggests that chi-recognition by RecBCD enzyme is accompanied by ejection of the RecD subunit.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
1356-9597
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
2
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
117-28
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:9167969-DNA,
pubmed-meshheading:9167969-DNA, Single-Stranded,
pubmed-meshheading:9167969-DNA Helicases,
pubmed-meshheading:9167969-Endodeoxyribonucleases,
pubmed-meshheading:9167969-Enzyme Activation,
pubmed-meshheading:9167969-Escherichia coli Proteins,
pubmed-meshheading:9167969-Exodeoxyribonuclease V,
pubmed-meshheading:9167969-Exodeoxyribonucleases,
pubmed-meshheading:9167969-Regulatory Sequences, Nucleic Acid
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pubmed:year |
1997
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pubmed:articleTitle |
Chi-activated RecBCD enzyme possesses 5'-->3' nucleolytic activity, but RecBC enzyme does not: evidence suggesting that the alteration induced by Chi is not simply ejection of the RecD subunit.
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pubmed:affiliation |
Genetics Graduate Group, University of California, Davis 95616-8665, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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