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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
1997-6-17
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pubmed:abstractText |
We characterized the activation of interleukin-1beta-converting enzyme (ICE)-like proteases (caspases) in human neuroblastoma cells (SH-SY5Y) following challenge with staurosporine, an established agent known to induce apoptosis. Time course analyses of lactate dehydrogenase release detected a significant increase in cell death as early as 6 h that continued at least until 24 h following staurosporine treatment. Western blot analyses using anti-poly(ADP-ribose) polymerase (anti-PARP) and anti-CPP32 antibodies revealed proteolytic processing of CPP32 (an ICE homologue) as well as fragmentation of PARP as early as 3 h following staurosporine challenge. Furthermore, the hydrolysis of the CPP32 substrate acetyl-DEVD-7-amido-4-methylcoumarin was detected as early as 3 h and became maximal at 6 h after staurosporine challenge, suggesting a delayed and sustained period of CPP32-like activation. In addition, we used the first immunohistochemical examination of CPP32 and PARP in cells following an apoptotic challenge. The localization of CPP32 in untreated SH-SY5Y cells was exclusively restricted to the cytoplasm. Following staurosporine challenge there was a condensing of CPP32 immunofluorescence from the cytoplasm to a region adjacent to the plasma membrane. In contrast, PARP immunofluorescence was evenly distributed in the nucleus in untreated SH-SY5Y cells and on staurosporine challenge was found to be associated with condensed chromatin. It is important that a pan ICE inhibitor [carbobenzoxy-Asp-CH2OC(O)-2,6-dichlorobenzene] was able to attenuate lactate dehydrogenase release and PARP and CPP32 cleavage and altered immunohistochemical staining patterns for PARP and CPP32 following staurosporine challenge.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Aspartic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/CASP3 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Caspase 1,
http://linkedlifedata.com/resource/pubmed/chemical/Caspase 3,
http://linkedlifedata.com/resource/pubmed/chemical/Caspases,
http://linkedlifedata.com/resource/pubmed/chemical/Coumarins,
http://linkedlifedata.com/resource/pubmed/chemical/Cysteine Endopeptidases,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Precursors,
http://linkedlifedata.com/resource/pubmed/chemical/Fluorescent Dyes,
http://linkedlifedata.com/resource/pubmed/chemical/Oligopeptides,
http://linkedlifedata.com/resource/pubmed/chemical/Poly(ADP-ribose) Polymerases,
http://linkedlifedata.com/resource/pubmed/chemical/Protease Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Staurosporine,
http://linkedlifedata.com/resource/pubmed/chemical/acetyl-aspartyl-glutamyl-valyl-aspar...,
http://linkedlifedata.com/resource/pubmed/chemical/benzyloxycarbonyl-Asp-CH2OC(O)-2,6-d...
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0022-3042
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
68
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2328-37
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:9166725-Antibody Specificity,
pubmed-meshheading:9166725-Apoptosis,
pubmed-meshheading:9166725-Aspartic Acid,
pubmed-meshheading:9166725-Caspase 1,
pubmed-meshheading:9166725-Caspase 3,
pubmed-meshheading:9166725-Caspases,
pubmed-meshheading:9166725-Coumarins,
pubmed-meshheading:9166725-Cysteine Endopeptidases,
pubmed-meshheading:9166725-DNA,
pubmed-meshheading:9166725-Enzyme Inhibitors,
pubmed-meshheading:9166725-Enzyme Precursors,
pubmed-meshheading:9166725-Fluorescent Antibody Technique,
pubmed-meshheading:9166725-Fluorescent Dyes,
pubmed-meshheading:9166725-Humans,
pubmed-meshheading:9166725-Hydrolysis,
pubmed-meshheading:9166725-Neuroblastoma,
pubmed-meshheading:9166725-Neurons,
pubmed-meshheading:9166725-Oligopeptides,
pubmed-meshheading:9166725-Poly(ADP-ribose) Polymerases,
pubmed-meshheading:9166725-Protease Inhibitors,
pubmed-meshheading:9166725-Staurosporine,
pubmed-meshheading:9166725-Tumor Cells, Cultured
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pubmed:year |
1997
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pubmed:articleTitle |
Characterization of CPP32-like protease activity following apoptotic challenge in SH-SY5Y neuroblastoma cells.
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pubmed:affiliation |
Department of Immunopathology, Parke-Davis Pharmaceutical Research, Warner-Lambert Company, Ann Arbor, Michigan 48105, U.S.A.
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pubmed:publicationType |
Journal Article
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