Linked Life Data

9165020

Source:http://linkedlifedata.com/resource/pubmed/id/9165020

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1997-6-17
pubmed:abstractText
In situ hybridization histochemistry was used to monitor the expression of 3beta-hydroxysteroid dehydrogenase, delta4-isomerase (3betaHSD) and cytochrome P450 11beta-hydroxylase (P45011beta) messenger RNA (mRNA) in adult rat adrenals after stimulation in vivo. In Exp 1, adrenals were collected from rats injected with saline or ACTH for 1, 2, 3, or 4 days. Adrenal sections from saline-treated rats showed uniform expression of 3betaHSD mRNA that extended from the adrenal capsule to the medullary border. In contrast, P45011beta mRNA showed high levels in the outer fasciculata and low levels in the inner fasciculata/reticularis. In response to ACTH, the integrated density of 3betaHSD hybridization did not increase until 4 days. The integrated density of P45011beta hybridization increased in ACTH-treated rats between 1-4 days due to increased hybridization in the inner fasciculata/reticularis. In Exp 2, rats were treated with ACTH or saline, and adrenals were harvested at 4, 8, or 24 h. The hybridization density of 3betaHSD did not change after ACTH or saline injection. Increased expression of P45011beta mRNA was observed at 4 and 8 h, but not 24 h post-ACTH. In Exp 3, to determine the response to acute stress, adrenals were collected from rats 24 h after surgical laparotomy. The integrated density of 3betaHSD labeling did not change, whereas both hybridization area and mean density of P45011beta increased. Increased expression of P45011beta mRNA was observed in the inner fasciculata similar to that observed after ACTH injection. In addition, adrenal cells were more responsive to ACTH in vitro after surgical stress. These results suggest that the rat adrenal cortex can respond to acute stress by up-regulation of the expression of steroidogenic enzyme genes and that this occurs in part by increasing the number of cells actively expressing P45011beta mRNA. The adrenal response after stress most likely results at least in part from stimulation by ACTH. These findings suggest that changes in adrenal steroidogenesis in response to ACTH may result from recruitment of steroidogenic cells to synthesize and secrete corticosteroids.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0013-7227
pubmed:author
pubmed:issnType
Print
pubmed:volume
138
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2338-46
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:9165020-3-Hydroxysteroid Dehydrogenases, pubmed-meshheading:9165020-Adrenal Cortex, pubmed-meshheading:9165020-Adrenal Medulla, pubmed-meshheading:9165020-Adrenocorticotropic Hormone, pubmed-meshheading:9165020-Animals, pubmed-meshheading:9165020-Drug Administration Schedule, pubmed-meshheading:9165020-Gene Expression Regulation, Enzymologic, pubmed-meshheading:9165020-In Situ Hybridization, pubmed-meshheading:9165020-Kinetics, pubmed-meshheading:9165020-Male, pubmed-meshheading:9165020-RNA, Messenger, pubmed-meshheading:9165020-Rats, pubmed-meshheading:9165020-Rats, Sprague-Dawley, pubmed-meshheading:9165020-Steroid 11-beta-Hydroxylase, pubmed-meshheading:9165020-Stress, Physiological, pubmed-meshheading:9165020-Time Factors, pubmed-meshheading:9165020-Transcription, Genetic
pubmed:year
1997
pubmed:articleTitle
Differential gene expression of cytochrome P450 11beta-hydroxylase in rat adrenal cortex after in vivo activation.
pubmed:affiliation
Department of Surgery, University of Minnesota, Minneapolis 55455, USA. engel002@tc.umn.edu
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't