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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
5
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pubmed:dateCreated |
1997-6-12
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pubmed:abstractText |
Urinary mutagenicity has been used in occupational and epidemiological studies for over two decades as a cost-effective, general biomarker of exposure to genotoxic agents. However, few studies have compared urinary mutagenicity to additional biomarkers determined among low- and high-exposed groups. To address this issue, we evaluated the relationship between urinary mutagenicity and other types of biomarkers in a cross-sectional study involving 15 workers exposed to the urinary bladder carcinogen benzidine (BZ, high exposure), 15 workers exposed to BZ-dyes (low exposure), and 13 unexposed controls in Ahmedabad, India. Urinary organics were extracted by C18/methanol and evaluated for mutagenicity in the presence of S9 in the Salmonella strain YG1024, which is a frameshift strain that overproduces acetyltransferase. The results were compared to biomarker data reported recently from the same urine samples (Rothman et al., Proc. Natl Acad. Sci. USA, 93, 5084-5089, 1996) that included a metabolite biomarker (the sum of the urinary levels of BZ + N-acetylbenzidine + N,N'-diacetylbenzidine) and a DNA adduct biomarker [a presumptive N-(3'-phosphodeoxyguanosin-8-yl)-N'-acetylbenzidine (C8dG-ABZ) DNA adduct in exfoliated urothelial cells]. The mean +/- SE urinary mutagenicity (revertants/micromol of creatinine) of the low-exposure (BZ-dye) workers was 8.2 +/- 2.4, which was significantly different from the mean of the controls (2.8 +/- 0.7, P = 0.04) as was that of the mean of the high-exposure (BZ) workers (123.2 +/- 26.1, P < 0.0001). Urinary mutagenicity showed strong, positive correlations with urinary metabolites (r = 0.88, P < 0.0001) and the level of the presumptive C8dG-ABZ urothelial DNA adduct (r = 0.59, P = 0.0006). A strong association was found between tobacco use (bidi smoking) and urinary mutagenicity among the controls (r = 0.68, P = 0.01) but not among the exposed workers (r = 0.18, P = 0.11). This study confirms the ability of a biomarker such as urinary mutagenicity to detect low-dose exposures, identify additional genotoxic exposures among the controls, and correlate strongly with urinary metabolites and DNA adducts in the target tissue (urinary bladder epithelia) in humans.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0143-3334
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pubmed:author |
pubmed-author:BhatnagarV KVK,
pubmed-author:BrooksL RLR,
pubmed-author:DavisB BBB,
pubmed-author:DeMariniD MDM,
pubmed-author:DosemeciMM,
pubmed-author:EischenB TBT,
pubmed-author:HayesR BRB,
pubmed-author:IVETT,
pubmed-author:JaegerMM,
pubmed-author:KashyapRR,
pubmed-author:KashyapS KSK,
pubmed-author:LakshmiVV,
pubmed-author:ParikhD JDJ,
pubmed-author:RothmanNN,
pubmed-author:SheltonM LML,
pubmed-author:TalaskaGG,
pubmed-author:ZenserT VTV
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pubmed:issnType |
Print
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pubmed:volume |
18
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
981-8
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pubmed:dateRevised |
2007-12-13
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pubmed:meshHeading |
pubmed-meshheading:9163684-Benzidines,
pubmed-meshheading:9163684-Biological Markers,
pubmed-meshheading:9163684-DNA Adducts,
pubmed-meshheading:9163684-Humans,
pubmed-meshheading:9163684-Male,
pubmed-meshheading:9163684-Mutagenicity Tests,
pubmed-meshheading:9163684-Mutagens,
pubmed-meshheading:9163684-Occupational Exposure,
pubmed-meshheading:9163684-Salmonella typhimurium,
pubmed-meshheading:9163684-Urine,
pubmed-meshheading:9163684-Urothelium
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pubmed:year |
1997
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pubmed:articleTitle |
Urinary mutagenicity as a biomarker in workers exposed to benzidine: correlation with urinary metabolites and urothelial DNA adducts.
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pubmed:affiliation |
Environmental Carcinogenesis Division, US Environmental Protection Agency, Research Triangle Park, NC 27711, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
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