Linked Life Data

9160882

Source:http://linkedlifedata.com/resource/pubmed/id/9160882

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
17
pubmed:dateCreated
1997-6-16
pubmed:abstractText
Like most other normal cells, human endothelial cells possess a limited replicative life span, and, after multiple passages in vitro, develop an arrest in cell division referred to as replicative senescence. For many cell types senescence can be delayed by oncogenes or tumor suppressor genes or prevented altogether by malignant transformation; however, once developed, senescence has been regarded as irreversible. We now report that a cytokine, vascular permeability factor/vascular endothelial growth factor (VPF/VEGF), significantly delays senescence in human dermal microvascular endothelial cells (HDMEC). Typically, VPF/VEGF-treated HDMEC could be cultured for at least 15-20 more population doublings (PD) than control cells. Protection from senescence was reversible in that subsequent withdrawal of VPF/VEGF returned cells to the senescent phenotype. Expression of several cell cycle-related genes (p21, p16 and p27) was significantly reduced in VPF/VEGF-treated cells but p53 expression was not significantly altered. Of particular importance, VPF/VEGF was able to rescue senescent HDMEC, restoring them to proliferation, to a more normal morphology, and to reduced expression of a senescence marker, neutral beta-galactosidase. Taken together, VPF/VEGF delayed the onset of senescence and also reversed senescence in microvascular endothelial cells without inducing cell transformation.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0950-9232
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
14
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2025-32
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:9160882-Brain-Derived Neurotrophic Factor, pubmed-meshheading:9160882-Capillaries, pubmed-meshheading:9160882-Cell Aging, pubmed-meshheading:9160882-Cell Division, pubmed-meshheading:9160882-Cell Size, pubmed-meshheading:9160882-Cells, Cultured, pubmed-meshheading:9160882-Depression, Chemical, pubmed-meshheading:9160882-Endothelial Growth Factors, pubmed-meshheading:9160882-Endothelium, Vascular, pubmed-meshheading:9160882-Gene Expression Regulation, pubmed-meshheading:9160882-Humans, pubmed-meshheading:9160882-Lymphokines, pubmed-meshheading:9160882-Male, pubmed-meshheading:9160882-Nerve Tissue Proteins, pubmed-meshheading:9160882-Recombinant Proteins, pubmed-meshheading:9160882-Skin, pubmed-meshheading:9160882-Vascular Endothelial Growth Factor A, pubmed-meshheading:9160882-Vascular Endothelial Growth Factors
pubmed:year
1997
pubmed:articleTitle
Vascular permeability factor/vascular endothelial growth factor (VPF/VEGF) delays and induces escape from senescence in human dermal microvascular endothelial cells.
pubmed:affiliation
Department of Pathology, Beth Israel Hospital and Harvard Medical School, Boston, Massachusetts 02215, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.