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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1997-7-14
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pubmed:databankReference |
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pubmed:abstractText |
Chlamydomonas reinhardtii, a unicellular green alga, grows photoautotrophically at very low concentrations of inorganic carbon due to the presence of an inducible CO2-concentrating mechanism. During the induction of the CO2-concentrating mechanism at low-CO2 growth conditions, at least five polypeptides that are either absent or present in low amounts in cells grown on high-CO2 concentrations are induced. One of these induced polypeptides with a molecular mass of 36 kD, LIP-36, has been localized to the chloroplast envelope. The protein was purified and the partial internal amino acid sequences were obtained through lys-C digestion. Two cDNAs encoding LIP-36 have been cloned using degenerate primers based on the amino acid sequences. The two genes encoding LIP-36 are highly homologous in the coding region but are completely different in the 5'-end and 3'-end untranslated regions. The deduced protein sequences show strong homology to the mitochondrial carrier protein superfamily, suggesting that LIP-36 is a chloroplast carrier protein. The regulation of the expression of these two genes at high- and low-CO2 growth conditions is also different. Both genes were highly expressed under low-CO2 growth conditions, with the steady-state level of LIP-36 G1 mRNA more abundant. However, neither gene was expressed at high-CO2 growth conditions. The gene products of both clones expressed in Escherichia coli were recognized by an antibody raised against LIP-36, confirming that the two cDNAs indeed encode the C. reinhardtii chloroplast envelope carrier protein LIP-36.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/9159951-16593518,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9159951-16661446,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9159951-16666333,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9159951-16666503,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9159951-16666639,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9159951-16667421,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9159951-16667546,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9159951-16668587,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9159951-1668652,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9159951-2112252,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9159951-2404796,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9159951-2714257,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9159951-6288471,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9159951-7523392,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9159951-7873543,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9159951-7876161,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9159951-8234257,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9159951-8291088
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0032-0889
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
114
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
265-73
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:9159951-Amino Acid Sequence,
pubmed-meshheading:9159951-Animals,
pubmed-meshheading:9159951-Base Sequence,
pubmed-meshheading:9159951-Carbon Dioxide,
pubmed-meshheading:9159951-Carrier Proteins,
pubmed-meshheading:9159951-Chlamydomonas reinhardtii,
pubmed-meshheading:9159951-Chloroplast Proteins,
pubmed-meshheading:9159951-Chloroplasts,
pubmed-meshheading:9159951-Cloning, Molecular,
pubmed-meshheading:9159951-DNA, Complementary,
pubmed-meshheading:9159951-DNA, Protozoan,
pubmed-meshheading:9159951-DNA Primers,
pubmed-meshheading:9159951-Escherichia coli,
pubmed-meshheading:9159951-Gene Expression Regulation,
pubmed-meshheading:9159951-Genes, Protozoan,
pubmed-meshheading:9159951-Molecular Sequence Data,
pubmed-meshheading:9159951-Protozoan Proteins,
pubmed-meshheading:9159951-Sequence Homology, Amino Acid
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pubmed:year |
1997
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pubmed:articleTitle |
Cloning and overexpression of two cDNAs encoding the low-CO2-inducible chloroplast envelope protein LIP-36 from Chlamydomonas reinhardtii.
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pubmed:affiliation |
Department of Plant Biology, Louisiana State University, Baton Rouge 70803, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.
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