Linked Life Data

9159889

Source:http://linkedlifedata.com/resource/pubmed/id/9159889

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1997-6-30
pubmed:abstractText
The role of 24:5n-3 and 24:6n-3 as intermediate in the formation of 22:6n-3 in trout liver was examined. Microsomes prepared from trout liver converted [1-14C]-eicosapentaenoic acid (20:5n-3) to 24: 5n-3 and 24:6n-3 but not docosahexaenoic acid (22:6n-3). The radiolabeled 24:5n-3 and 24:6n-3 were isolated from the microsomal incubations by argentation chromatography and used as substrates in incubations with hepatocytes isolated from trout liver. Both 14C-labelled 24:6n-3 and 22:6n-3-were produced by hepatocytes incubated with radiolabelled 24:5n-3. When hepatocytes were incubated with radiolabelled 24:6n-3, the amount of radioactivity recovered in 22:6n-3 over 6 hr increased in direct relation to the decrease observed in the amount of radioactivity recovered in 24:6n-3. The results suggest that the formation of 22:6n-3 in trout liver does not involve delta 4 desaturation of 22:5n-3 but rather proceeds via the delta 6 desaturation of 24:5n-3 with the subsequent chain shortening of the 24:6n-3 produced.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
1096-4959
pubmed:author
pubmed:issnType
Print
pubmed:volume
116
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
263-7
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1997
pubmed:articleTitle
Biosynthesis of docosahexaenoic acid in trout hepatocytes proceeds via 24-carbon intermediates.
pubmed:affiliation
NERC Unit of Aquatic Biochemistry, Department of Molecular and Biological Sciences, University of Stirling, Scotland, U.K.
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't