| pubmed-article:9159281 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9159281 | lifeskim:mentions | umls-concept:C0010453 | lld:lifeskim |
| pubmed-article:9159281 | lifeskim:mentions | umls-concept:C0007452 | lld:lifeskim |
| pubmed-article:9159281 | lifeskim:mentions | umls-concept:C0324533 | lld:lifeskim |
| pubmed-article:9159281 | lifeskim:mentions | umls-concept:C0013935 | lld:lifeskim |
| pubmed-article:9159281 | lifeskim:mentions | umls-concept:C0162867 | lld:lifeskim |
| pubmed-article:9159281 | lifeskim:mentions | umls-concept:C1882726 | lld:lifeskim |
| pubmed-article:9159281 | lifeskim:mentions | umls-concept:C0125606 | lld:lifeskim |
| pubmed-article:9159281 | pubmed:issue | 5 | lld:pubmed |
| pubmed-article:9159281 | pubmed:dateCreated | 1997-6-19 | lld:pubmed |
| pubmed-article:9159281 | pubmed:abstractText | Experiments were designed to study development of bovine embryos in TCM-199 medium conditioned by preculture with buffalo rat liver (BRL) cells. Conditioned media were harvested after BRL cells were cultured until confluency (CON), or for an additional 2 d with the same cells but new medium (CON-N) or the same medium (CON-S). Glucose in TCM-199 was depleted by BRL cells to different concentrations depending on coculture procedures: CON = 3.94 mM, CON-N = 1.67 mM, and CON-S = 1.11 mM glucose. In Exp. 1, development of bovine zygotes in CON-S resulted in fewer blastocysts than development in CON (10 vs 28%, P < .05); CON-N was not different from CON (26% blastocysts). Experiment 2 examined effects of moving embryos to a fresh drop of different or identical conditioned medium after culture for 3 d. Initial culture in CON-N and final culture in CON resulted in a greater (P < .01) number of blastocysts compared with the control of CON followed by CON (32 vs 19% blastocysts). This was not entirely due to changing from low to high glucose because adding glucose to CON-N after 3 d yielded only 18% blastocysts. To test the hypothesis that beneficial effects of BRL cell-conditioned media may be due to secretion of leukemia inhibiting factor (LIF), LIF was added to B2, a more appropriate medium than Medium-199 for culturing bovine embryos without conditioning or coculture with BRL cells. In the absence of serum, the percentage of blastocysts per cleaved embryo (17 to 28%) was not improved with LIF; however, the mean number of cells per blastocyst was higher (P < .05) in treatments with LIF (65 to 74 cells) than without LIF (47 cells). In B2 medium + 10% fetal calf serum, LIF was of no benefit; development to blastocysts was good with or without LIF (43% of cleaved). | lld:pubmed |
| pubmed-article:9159281 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9159281 | pubmed:language | eng | lld:pubmed |
| pubmed-article:9159281 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9159281 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:9159281 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9159281 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9159281 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9159281 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9159281 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9159281 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9159281 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9159281 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9159281 | pubmed:month | May | lld:pubmed |
| pubmed-article:9159281 | pubmed:issn | 0021-8812 | lld:pubmed |
| pubmed-article:9159281 | pubmed:author | pubmed-author:SeidelG EGEJr | lld:pubmed |
| pubmed-article:9159281 | pubmed:author | pubmed-author:NautaW JWJ | lld:pubmed |
| pubmed-article:9159281 | pubmed:author | pubmed-author:FunstonR NRN | lld:pubmed |
| pubmed-article:9159281 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9159281 | pubmed:volume | 75 | lld:pubmed |
| pubmed-article:9159281 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:9159281 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:9159281 | pubmed:pagination | 1332-6 | lld:pubmed |
| pubmed-article:9159281 | pubmed:dateRevised | 2007-11-15 | lld:pubmed |
| pubmed-article:9159281 | pubmed:meshHeading | pubmed-meshheading:9159281-... | lld:pubmed |
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| pubmed-article:9159281 | pubmed:meshHeading | pubmed-meshheading:9159281-... | lld:pubmed |
| pubmed-article:9159281 | pubmed:year | 1997 | lld:pubmed |
| pubmed-article:9159281 | pubmed:articleTitle | Culture of bovine embryos in buffalo rat liver cell-conditioned media or with leukemia inhibitory factor. | lld:pubmed |
| pubmed-article:9159281 | pubmed:affiliation | Animal Reproduction and Biotechnology Laboratory, Colorado State University, Fort Collins 80523, USA. | lld:pubmed |
| pubmed-article:9159281 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:9159281 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:9159281 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
