Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
1997-6-19
pubmed:abstractText
The familial breast-ovarian tumor suppressor gene product BRCA1 was found to be a component of the RNA polymerase II holoenzyme by several criteria. BRCA1 was found to copurify with the holoenzyme over multiple chromatographic steps. Other tested transcription activators that could potentially contact the holoenzyme were not stably associated with the holoenzyme as determined by copurification. Antibody specific for the holoenzyme component hSRB7 specifically purifies BRCA1. Immunopurification of BRCA1 complexes also specifically purifies transcriptionally active RNA polymerase II and transcription factors TFIIF, TFIIE, and TFIIH. Moreover, a BRCA1 domain, which is deleted in about 90% of clinically relevant mutations, participates in binding to the holoenzyme complex in cells. These data are consistent with recent data identifying transcription activation domains in the BRCA1 protein and link the BRCA1 tumor suppressor protein with the transcription process as a holoenzyme-bound protein.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/9159119-1303261, http://linkedlifedata.com/resource/pubmed/commentcorrection/9159119-1720509, http://linkedlifedata.com/resource/pubmed/commentcorrection/9159119-1956373, http://linkedlifedata.com/resource/pubmed/commentcorrection/9159119-2324114, http://linkedlifedata.com/resource/pubmed/commentcorrection/9159119-6193397, http://linkedlifedata.com/resource/pubmed/commentcorrection/9159119-7545954, http://linkedlifedata.com/resource/pubmed/commentcorrection/9159119-7553866, http://linkedlifedata.com/resource/pubmed/commentcorrection/9159119-7568034, http://linkedlifedata.com/resource/pubmed/commentcorrection/9159119-7707539, http://linkedlifedata.com/resource/pubmed/commentcorrection/9159119-7736588, http://linkedlifedata.com/resource/pubmed/commentcorrection/9159119-7753848, http://linkedlifedata.com/resource/pubmed/commentcorrection/9159119-7758944, http://linkedlifedata.com/resource/pubmed/commentcorrection/9159119-7877695, http://linkedlifedata.com/resource/pubmed/commentcorrection/9159119-7939630, http://linkedlifedata.com/resource/pubmed/commentcorrection/9159119-7954448, http://linkedlifedata.com/resource/pubmed/commentcorrection/9159119-8133894, http://linkedlifedata.com/resource/pubmed/commentcorrection/9159119-8187178, http://linkedlifedata.com/resource/pubmed/commentcorrection/9159119-8541881, http://linkedlifedata.com/resource/pubmed/commentcorrection/9159119-8565069, http://linkedlifedata.com/resource/pubmed/commentcorrection/9159119-8598913, http://linkedlifedata.com/resource/pubmed/commentcorrection/9159119-8600523, http://linkedlifedata.com/resource/pubmed/commentcorrection/9159119-8609996, http://linkedlifedata.com/resource/pubmed/commentcorrection/9159119-8700522, http://linkedlifedata.com/resource/pubmed/commentcorrection/9159119-8751436, http://linkedlifedata.com/resource/pubmed/commentcorrection/9159119-8779723, http://linkedlifedata.com/resource/pubmed/commentcorrection/9159119-8824594, http://linkedlifedata.com/resource/pubmed/commentcorrection/9159119-8848048, http://linkedlifedata.com/resource/pubmed/commentcorrection/9159119-8876171, http://linkedlifedata.com/resource/pubmed/commentcorrection/9159119-8942979, http://linkedlifedata.com/resource/pubmed/commentcorrection/9159119-9008167, http://linkedlifedata.com/resource/pubmed/commentcorrection/9159119-9087428, http://linkedlifedata.com/resource/pubmed/commentcorrection/9159119-9096323
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:day
27
pubmed:volume
94
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
5605-10
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1997
pubmed:articleTitle
BRCA1 is a component of the RNA polymerase II holoenzyme.
pubmed:affiliation
Dana-Farber Cancer Institute, 44 Binney Street, Boston, MA 02115;
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't