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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1997-7-21
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pubmed:abstractText |
We examined the expression of the murine homologue of myotonic dystrophy associated homeodomain protein (mDMAHP) using two different strategies. The first approach, RT-PCR, detected spliced transcripts in a wide range of embryonic and adult tissues, in a pattern overlapping substantially with the expression of mDMPK. A second approach, the generation of transgenic mice expressing the lacZ reporter gene from a 4.3 kb promoter fragment, also demonstrated expression in a range of tissues with potential links to the phenotype in myotonic dystrophy. We conclude that murine DMAHP has a similar pattern of expression to human DMAHP and will serve as a useful model for functional studies of this gene, although species differences, such as the reduced CpG island (1.8 kb compared with 3.5 kb) must be carefully considered.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0964-6906
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
6
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
651-7
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pubmed:dateRevised |
2009-9-29
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pubmed:meshHeading |
pubmed-meshheading:9158137-Age Factors,
pubmed-meshheading:9158137-Amino Acid Sequence,
pubmed-meshheading:9158137-Animals,
pubmed-meshheading:9158137-Base Sequence,
pubmed-meshheading:9158137-CpG Islands,
pubmed-meshheading:9158137-Embryo, Mammalian,
pubmed-meshheading:9158137-Female,
pubmed-meshheading:9158137-Gene Expression Regulation, Developmental,
pubmed-meshheading:9158137-Genes, Reporter,
pubmed-meshheading:9158137-Homeodomain Proteins,
pubmed-meshheading:9158137-Humans,
pubmed-meshheading:9158137-Male,
pubmed-meshheading:9158137-Mice,
pubmed-meshheading:9158137-Mice, Inbred C57BL,
pubmed-meshheading:9158137-Mice, Inbred CBA,
pubmed-meshheading:9158137-Mice, Transgenic,
pubmed-meshheading:9158137-Molecular Sequence Data,
pubmed-meshheading:9158137-Myotonic Dystrophy,
pubmed-meshheading:9158137-Polymerase Chain Reaction,
pubmed-meshheading:9158137-Pregnancy,
pubmed-meshheading:9158137-RNA, Messenger,
pubmed-meshheading:9158137-RNA Splicing,
pubmed-meshheading:9158137-Recombinant Fusion Proteins,
pubmed-meshheading:9158137-Sequence Homology, Amino Acid,
pubmed-meshheading:9158137-Transcription, Genetic,
pubmed-meshheading:9158137-beta-Galactosidase
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pubmed:year |
1997
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pubmed:articleTitle |
Characterisation of expression of mDMAHP, a homeodomain-encoding gene at the murine DM locus.
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pubmed:affiliation |
Department of Pharmacology, Charing Cross & Westminster Medical School, London, UK.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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