Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1997-6-19
pubmed:abstractText
In cooperation with an activated ras oncogene, the site-dependent AP-1 transcription factor c-Jun transforms primary rat embryo fibroblasts (REF). Although signal transduction pathways leading to activation of c-Jun proteins have been extensively studied, little is known about c-Jun cellular targets. We identified c-Jun-upregulated cDNA clones homologous to the tenascin-C gene by differential screening of a cDNA library from REF. This tightly regulated gene encodes a rare extracellular matrix protein involved in cell attachment and migration and in the control of cell growth. Transient overexpression of c-Jun induced tenascin-C expression in primary REF and in FR3T3, an established fibroblast cell line. Surprisingly, tenascin-C synthesis was repressed after stable transformation by c-Jun compared to that in the nontransformed parental cells. As assessed by using the tenascin-C (-220 to +79) promoter fragment cloned in a reporter construct, the c-Jun-induced transient activation is mediated by two binding sites: one GCN4/AP-1-like site, at position -146, and one NF-kappaB site, at position -210. Furthermore, as demonstrated by gel shift experiments and cotransfections of the reporter plasmid and expression vectors encoding the p65 subunit of NF-kappaB and c-Jun, the two transcription factors bind and synergistically transactivate the tenascin-C promoter. We previously described two other extracellular matrix proteins, SPARC and thrombospondin-1, as c-Jun targets. Thus, our results strongly suggest that the regulation of the extracellular matrix composition plays a central role in c-Jun-induced transformation.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/9154819-1310896, http://linkedlifedata.com/resource/pubmed/commentcorrection/9154819-1371773, http://linkedlifedata.com/resource/pubmed/commentcorrection/9154819-1374030, http://linkedlifedata.com/resource/pubmed/commentcorrection/9154819-1383086, http://linkedlifedata.com/resource/pubmed/commentcorrection/9154819-1383239, http://linkedlifedata.com/resource/pubmed/commentcorrection/9154819-1385122, http://linkedlifedata.com/resource/pubmed/commentcorrection/9154819-1385335, http://linkedlifedata.com/resource/pubmed/commentcorrection/9154819-1385427, http://linkedlifedata.com/resource/pubmed/commentcorrection/9154819-1459456, http://linkedlifedata.com/resource/pubmed/commentcorrection/9154819-1682056, http://linkedlifedata.com/resource/pubmed/commentcorrection/9154819-1710226, http://linkedlifedata.com/resource/pubmed/commentcorrection/9154819-1717349, http://linkedlifedata.com/resource/pubmed/commentcorrection/9154819-1722330, http://linkedlifedata.com/resource/pubmed/commentcorrection/9154819-1723942, http://linkedlifedata.com/resource/pubmed/commentcorrection/9154819-1749429, http://linkedlifedata.com/resource/pubmed/commentcorrection/9154819-1751545, http://linkedlifedata.com/resource/pubmed/commentcorrection/9154819-1846781, http://linkedlifedata.com/resource/pubmed/commentcorrection/9154819-1903181, http://linkedlifedata.com/resource/pubmed/commentcorrection/9154819-1935902, http://linkedlifedata.com/resource/pubmed/commentcorrection/9154819-1944289, http://linkedlifedata.com/resource/pubmed/commentcorrection/9154819-2123464, http://linkedlifedata.com/resource/pubmed/commentcorrection/9154819-2123532, http://linkedlifedata.com/resource/pubmed/commentcorrection/9154819-2166997, http://linkedlifedata.com/resource/pubmed/commentcorrection/9154819-2169352, http://linkedlifedata.com/resource/pubmed/commentcorrection/9154819-2203531, http://linkedlifedata.com/resource/pubmed/commentcorrection/9154819-2428505, http://linkedlifedata.com/resource/pubmed/commentcorrection/9154819-2440026, http://linkedlifedata.com/resource/pubmed/commentcorrection/9154819-2440339, http://linkedlifedata.com/resource/pubmed/commentcorrection/9154819-2462568, http://linkedlifedata.com/resource/pubmed/commentcorrection/9154819-2480799, http://linkedlifedata.com/resource/pubmed/commentcorrection/9154819-2648396, http://linkedlifedata.com/resource/pubmed/commentcorrection/9154819-2825349, http://linkedlifedata.com/resource/pubmed/commentcorrection/9154819-3034432, http://linkedlifedata.com/resource/pubmed/commentcorrection/9154819-3037355, http://linkedlifedata.com/resource/pubmed/commentcorrection/9154819-3040261, http://linkedlifedata.com/resource/pubmed/commentcorrection/9154819-3135940, http://linkedlifedata.com/resource/pubmed/commentcorrection/9154819-3464968, http://linkedlifedata.com/resource/pubmed/commentcorrection/9154819-7481820, http://linkedlifedata.com/resource/pubmed/commentcorrection/9154819-7505785, http://linkedlifedata.com/resource/pubmed/commentcorrection/9154819-7531707, http://linkedlifedata.com/resource/pubmed/commentcorrection/9154819-7534342, http://linkedlifedata.com/resource/pubmed/commentcorrection/9154819-7583121, http://linkedlifedata.com/resource/pubmed/commentcorrection/9154819-7622446, http://linkedlifedata.com/resource/pubmed/commentcorrection/9154819-7679097, http://linkedlifedata.com/resource/pubmed/commentcorrection/9154819-7685035, http://linkedlifedata.com/resource/pubmed/commentcorrection/9154819-7694605, http://linkedlifedata.com/resource/pubmed/commentcorrection/9154819-7748560, http://linkedlifedata.com/resource/pubmed/commentcorrection/9154819-7935449, http://linkedlifedata.com/resource/pubmed/commentcorrection/9154819-7988564, http://linkedlifedata.com/resource/pubmed/commentcorrection/9154819-8004669, http://linkedlifedata.com/resource/pubmed/commentcorrection/9154819-8404856, http://linkedlifedata.com/resource/pubmed/commentcorrection/9154819-8464749, http://linkedlifedata.com/resource/pubmed/commentcorrection/9154819-8628254
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0270-7306
pubmed:author
pubmed:issnType
Print
pubmed:volume
17
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3202-9
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
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