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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
11
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pubmed:dateCreated |
1997-7-9
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pubmed:abstractText |
We describe a method, complementary strand analysis (CSA), for separating alleles potentially from any heterozygous genetic locus. Locus specific PCR is performed generating two allelic products. The antisense strands are isolated and hybridised with a sense reference strand to form a chimeric DNA duplex for each allele which is then separated by non-denaturing PAGE. We demonstrate the application of CSA for separation of highly polymorphic HLA-A, -B and -Cw alleles and characterisation of HLA identity in related bone marrow donors and patients. CSA is capable of resolving one nucleotide differences in a DNA fragment nearly as large as a kilobase in length.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/9153329-1284534,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9153329-1701246,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9153329-2320591,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9153329-2565038,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9153329-2986011,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9153329-3969155,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9153329-6220406,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9153329-7687986,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9153329-7725305,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9153329-8855291,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9153329-9157086
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0305-1048
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
25
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2236-8
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:9153329-Alleles,
pubmed-meshheading:9153329-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:9153329-Female,
pubmed-meshheading:9153329-Genetic Complementation Test,
pubmed-meshheading:9153329-Genetic Techniques,
pubmed-meshheading:9153329-HLA-A Antigens,
pubmed-meshheading:9153329-HLA-B Antigens,
pubmed-meshheading:9153329-HLA-C Antigens,
pubmed-meshheading:9153329-Humans,
pubmed-meshheading:9153329-Male,
pubmed-meshheading:9153329-Polymerase Chain Reaction,
pubmed-meshheading:9153329-Polymorphism, Genetic
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pubmed:year |
1997
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pubmed:articleTitle |
Complementary strand analysis: a new approach for allelic separation in complex polyallelic genetic systems.
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pubmed:affiliation |
The Anthony Nolan Research Institute, The Royal Free Hospital, Pond Street, Hampstead, London NW3 2QG, UK.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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