Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
1997-7-9
pubmed:abstractText
We show that the requirement for Prp18 during the second step of actin pre-mRNA splicing in vitro is dictated by the distance between the branch point and the 3'splice site. Prp18 is dispensable for splicing of precursor RNAs in which the interval between the branch point and 3'splice site is <12 nt. This resembles the requirement for another second step factor, Slu7. Excess Slu7 protein can bypass the need for Prp18 in vitro , suggesting that Slu7 and Prp18 function in a concerted manner. Physical interaction between Slu7 and Prp18 was demonstrated by using the two-hybrid assay. Deletion mutants of SLU7 were tested for their ability to support growth of a slu7 null strain. Removal of 199 amino acids from the N-terminus of the 382 amino acid Slu7 protein did not affect cell viability at 25 degrees C. A more extensive N-terminal deletion of 221 amino acids was lethal, as was a C-terminal deletion of 47 amino acids. Deleted versions of Slu7 were also tested for interaction with Prp18 in the two-hybrid system. We define a segment of Slu7 from residue 200 to 224 that is necessary for interaction with Prp18.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/9153314-1406691, http://linkedlifedata.com/resource/pubmed/commentcorrection/9153314-1427075, http://linkedlifedata.com/resource/pubmed/commentcorrection/9153314-1464325, http://linkedlifedata.com/resource/pubmed/commentcorrection/9153314-1739979, http://linkedlifedata.com/resource/pubmed/commentcorrection/9153314-1825134, http://linkedlifedata.com/resource/pubmed/commentcorrection/9153314-1846089, http://linkedlifedata.com/resource/pubmed/commentcorrection/9153314-1853200, http://linkedlifedata.com/resource/pubmed/commentcorrection/9153314-2005795, http://linkedlifedata.com/resource/pubmed/commentcorrection/9153314-2031287, http://linkedlifedata.com/resource/pubmed/commentcorrection/9153314-2403639, http://linkedlifedata.com/resource/pubmed/commentcorrection/9153314-2547163, http://linkedlifedata.com/resource/pubmed/commentcorrection/9153314-2695083, http://linkedlifedata.com/resource/pubmed/commentcorrection/9153314-2997224, http://linkedlifedata.com/resource/pubmed/commentcorrection/9153314-3023896, http://linkedlifedata.com/resource/pubmed/commentcorrection/9153314-3305158, http://linkedlifedata.com/resource/pubmed/commentcorrection/9153314-3550418, http://linkedlifedata.com/resource/pubmed/commentcorrection/9153314-7489518, http://linkedlifedata.com/resource/pubmed/commentcorrection/9153314-7500330, http://linkedlifedata.com/resource/pubmed/commentcorrection/9153314-7534458, http://linkedlifedata.com/resource/pubmed/commentcorrection/9153314-7535718, http://linkedlifedata.com/resource/pubmed/commentcorrection/9153314-7568198, http://linkedlifedata.com/resource/pubmed/commentcorrection/9153314-7664739, http://linkedlifedata.com/resource/pubmed/commentcorrection/9153314-8242751, http://linkedlifedata.com/resource/pubmed/commentcorrection/9153314-8341661, http://linkedlifedata.com/resource/pubmed/commentcorrection/9153314-8397340, http://linkedlifedata.com/resource/pubmed/commentcorrection/9153314-8436300, http://linkedlifedata.com/resource/pubmed/commentcorrection/9153314-8474454, http://linkedlifedata.com/resource/pubmed/commentcorrection/9153314-8548652, http://linkedlifedata.com/resource/pubmed/commentcorrection/9153314-8725222, http://linkedlifedata.com/resource/pubmed/commentcorrection/9153314-8756413, http://linkedlifedata.com/resource/pubmed/commentcorrection/9153314-9000057
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0305-1048
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
25
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2146-52
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed:year
1997
pubmed:articleTitle
Functional and physical interaction between the yeast splicing factors Slu7 and Prp18.
pubmed:affiliation
Department of Biochemistry, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA.
pubmed:publicationType
Journal Article, In Vitro, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't