|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
6
|
|
pubmed:dateCreated |
1997-6-9
|
|
pubmed:abstractText |
Previous investigations into recombination in cowpea chlorotic mottle bromovirus (CCMV) resulted in the recovery of an unusual recombinant virus, 3-57, which caused a symptomless infection of cowpeas but formed no detectable virions. Sequence analysis of cDNA clones derived from 3-57 determined that mutations near the 5' terminus of the capsid protein gene introduced an early translational termination codon. Further mutations introduced a new in-frame start codon that allowed translation of the 3' two-thirds of the capsid protein gene. Based on the mutations observed in 3-57, wild-type CCMV clones were modified to determine if the carboxyl two-thirds of the capsid protein functions independently of the complete protein in long-distance movement. Analysis of these mutants determined that while virion formation is not required for systemic infection, the carboxy-terminal two-thirds of the capsid protein is both required and sufficient for systemic movement of viral RNA. This indicates that the CCMV capsid protein is multifunctional, with a distinct long-distance movement function in addition to its role in virion formation.
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/9151887-1472715,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9151887-1690559,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9151887-1727594,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9151887-1733093,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9151887-1905801,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9151887-2014641,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9151887-2053275,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9151887-2053278,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9151887-2308940,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9151887-2795712,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9151887-3418781,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9151887-4975324,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9151887-7743132,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9151887-7769722,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9151887-7772801,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9151887-7780307,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9151887-7856075,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9151887-8128222,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9151887-8517020,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9151887-911786
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Jun
|
|
pubmed:issn |
0022-538X
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
71
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
4862-5
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:9151887-Bromovirus,
pubmed-meshheading:9151887-Capsid,
pubmed-meshheading:9151887-DNA Mutational Analysis,
pubmed-meshheading:9151887-Fabaceae,
pubmed-meshheading:9151887-Gene Expression Regulation, Viral,
pubmed-meshheading:9151887-Genes, Viral,
pubmed-meshheading:9151887-Plant Viral Movement Proteins,
pubmed-meshheading:9151887-Plants, Medicinal,
pubmed-meshheading:9151887-Plants, Toxic,
pubmed-meshheading:9151887-RNA, Messenger,
pubmed-meshheading:9151887-Tobacco,
pubmed-meshheading:9151887-Viral Proteins,
pubmed-meshheading:9151887-Viral Structural Proteins,
pubmed-meshheading:9151887-Virion
|
|
pubmed:year |
1997
|
|
pubmed:articleTitle |
The carboxy-terminal two-thirds of the cowpea chlorotic mottle bromovirus capsid protein is incapable of virion formation yet supports systemic movement.
|
|
pubmed:affiliation |
Genetics Program, Michigan State University, East Lansing 48824, USA.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
